| 核桃组织培养中外植体材料的初代培养研究 |
| |
| 引用本文： | 张小红,闵东红,康冰,李军超,马勤.核桃组织培养中外植体材料的初代培养研究[J].陕西林业科技,2005(2):6-8. |
| |
| 作者姓名： | 张小红 闵东红 康冰 李军超 马勤 |
| |
| 作者单位： | 西北农林科技大学,陕西,杨凌,712100 |
| |
| 基金项目： | 陕西省自然科学基金项目（项目编号：2003C133）。 |
| |
| 摘 要： | 以3个核桃品种为试材，对初代培养中外植体的消毒方法、取材时间、培养基和外植体类型等因子对离体培养的影响进行了初步研究。结果表明：二次升汞消毒法可以提高休眠芽的消毒效果：以嫩枝茎段为外植体时适宜的取材暑期为6月，以腋芽饱满半木质化的培养效果较好；适宜的培养基类型是DKW和1/2MS，嫩枝茎段的培养效果要好于休眠芽；3个品种间离体培养特性存在差异。
|
| 关 键 词： | 核桃 外植体 初代培养 |
| 文章编号： | 1001-2117（2005）02-0006-03 |
| 收稿时间： | 2005-01-12 |
| 修稿时间： | 2005年1月12日 |
Primary Culture of Explants in Tissue Culture of Walnut |
| |
| ZHANG Xiao-hong,MIN Dong-hong,KANG Bing,LI Jun-chao,MA Qin.Primary Culture of Explants in Tissue Culture of Walnut[J].Shaanxi Forest Science and Technology,2005(2):6-8. |
| |
| Authors: | ZHANG Xiao-hong MIN Dong-hong KANG Bing LI Jun-chao MA Qin |
| |
| Abstract: | With three walnut cultivars as experiment materials, the influence of primary explants in different ways of sterilization, time of material-taking, and types of explants on vitro culture was studied. The result showed that two steps of sterilization by mercury improved sterilization effect for dormant buds; tender shoots were most suitable in June as explants material with full axilla buds and semi-lignification; tender shoots had better culture results than dormancy buds; and that DKW and 1/2MS media were both fitting for explants in vitro culture. Differences of culture characteristic among three cultivars were observed. |
| |
| Keywords: | Walnut explants primary culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
