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响应曲面法优化淫羊藿黄酮提取工艺
引用本文:林敏,张帆,吴冬青,安红钢,任雪峰.响应曲面法优化淫羊藿黄酮提取工艺[J].中兽医医药杂志,2012,31(6):16-19.
作者姓名:林敏  张帆  吴冬青  安红钢  任雪峰
作者单位:河西学院甘肃省高校河西走廊特色资源利用省级重点实验室,甘肃张掖,734000
基金项目:甘肃省教育厅研究生导师资助项目,甘肃省高校河西走廊特色资源利用省级重点实验室科研资助项目
摘    要:在单因素试验基础上,采用响应面法的(Box-Benhnken)组合设计原理,对淫羊藿总黄酮的提取工艺参数进行优化。淫羊藿总黄酮的最佳提取工艺条件为:提取时间1.73 h、水料比31∶1(mL/g),温度67.5℃、乙醇浓度79%,淫藿黄酮类化合物的平均提取率为2.054%,比理论预测值高0.08%。

关 键 词:淫羊藿  总黄酮  提取  响应面分析

Optimization of the extraction process for flavonoids in Herba Epimedii by response surface method
LIN Min,ZHANG Fan,WU Dong-qing,AN Hong-gang,REN Xue-feng.Optimization of the extraction process for flavonoids in Herba Epimedii by response surface method[J].Journal of Traditional Chinese Veterinary Medicine,2012,31(6):16-19.
Authors:LIN Min  ZHANG Fan  WU Dong-qing  AN Hong-gang  REN Xue-feng
Institution:(Key Laboratory of Hexi Corridor Resources Utilization of Gansu Universities, Hexi University, Zhangye Gansu 734000, China)
Abstract:On the basis of single factor experiment,the conditions for extracting flavonoids from Herba Epimedii were optimized with response surface method (RSM). The optimal conditions for flavones extraction were obtained as follows: extraction temperature of 67.5℃ ,water/solid ratio of 31, extraction time of 1.73 h,and ethanol concentration of 79%. Under the above conditions,the one-time extraction rate was up to 2.054%,which was 0.08% more than the theoretical value.
Keywords:Herba Epimedii  flavonoids  extraction  response surface experiment
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