| 水牛有腔卵泡颗粒细胞凋亡的生物形态学特征 |
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| 引用本文： | 何宝祥,郑喜邦,杜玉兰,余四九,李恭贺,李彩萍.水牛有腔卵泡颗粒细胞凋亡的生物形态学特征[J].畜牧兽医学报,2006,37(11):1209-1216. |
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| 作者姓名： | 何宝祥 郑喜邦 杜玉兰 余四九 李恭贺 李彩萍 |
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| 作者单位： | 1. 广西大学动物科技学院,南宁,530005
2. 甘肃农业大学动物医学院,兰州,730070 |
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| 基金项目： | 广西科学基金项目（桂基科0144009）;广西“新世纪十百千人才工程”专项资金（2001210号） |
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| 摘 要： | 为了解水牛有腔卵泡闭锁的起因和有腔卵泡中是否存在卵泡颗粒细胞凋亡，本研究选择青春期前水牛和成年（发情间期和发情期）水牛的卵巢，采用石蜡组织切片、HE染色技术进行光镜观察，并采用DNA原位末端标记（TUNEL）技术和透射电镜观察研究了卵泡颗粒细胞凋亡的特征。结果表明：青春期前水牛的健康有腔卵泡上无或仅有极个别凋亡的卵泡颗粒细胞（GCs），而闭锁有腔卵泡上存在有多量凋亡的GCs，凋亡的形式：GCs层内出现单个或多个凋亡细胞，卵泡腔内出现凋亡小体群。在成年水牛发情间期的闭锁大卵泡上，GCs层弯曲皱折，存在多量凋亡的GCs。发情期水牛成熟卵泡的GCs层也存在GCs凋亡，排卵前的GCs层细胞全部脱落到卵泡腔内。本研究首次在同一组织切片上先后分别应用HE染色和TUNEL检查细胞凋亡，且证实了水牛有腔卵泡GCs凋亡的存在。超微结构显示了GCs凋亡的核边集化、核浓缩及凋亡小体的形态。上述结果提示：在水牛闭锁有腔卵泡中存在着GCs凋亡，GCs凋亡是启动水牛有腔卵泡闭锁的潜在机理。
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| 关 键 词： | 水牛 细胞凋亡 透射电镜 卵泡颗粒细胞 TUNEL |
| 文章编号： | 0366-6964（2006）11-1209-08 |
| 收稿时间： | 2005-04-20 |
| 修稿时间： | 2005-04-20 |
The Biological Characteristics of Granulosa Cells Apoptosis of Antritic Follicle in Buffalo Ovary |
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| HE Bao-xiang,ZHENG Xi-bang,DU Yu-lan,YU Si-jiu,LI Gong-he,LI Cai-ping.The Biological Characteristics of Granulosa Cells Apoptosis of Antritic Follicle in Buffalo Ovary[J].Acta Veterinaria et Zootechnica Sinica,2006,37(11):1209-1216. |
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| Authors: | HE Bao-xiang ZHENG Xi-bang DU Yu-lan YU Si-jiu LI Gong-he LI Cai-ping |
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| Institution: | 1. College of Animal Science and Technology, Guangxi University, Nanning 530005, China ; 2. Animal Medicine College, Gansu Agricultural University ,Lanzhou 730070, China |
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| Abstract: | In ordor to reveal the origination of antral follicular atresia,and to understand if the apoptosis of granulosa cells(GCs) of antrum follicle exists in buffalo ovarian,the buffaloes before puberty and adult buffaloes(at oestrus & dioestrus) were chosen in this study,the apoptotic characteristics of follicular GCs was studied by paraffin section,HE staining,light microscope,DNA in situ end labeling(terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling,TUNEL) and transmit electronic microscope(TEM) technology.The results showed that the healthy antrum follicle had not or only had infrequent apoptotic GCs, while atretic antrum follicle had plentiful apoptotic GCs in buffalo before puberty,the representative forms were apoptosis of single or more GCs in GCs layer,and flock of apoptotic bodies in follicular cavity.There were many apoptotic GCs and the GCs layer was curving and crinkling in the big atretic follicle of adult buffalo at dioestrus.GCs apoptosis also exists in Graafian follicle of buffalo at estrus,all GCs in GCs layer fallen into follicular cavity in the big follicle before ovulation.A apoptosis-examining method of the same tissue slice being respectively stained by HE staining and TUNEL has been invented,GCs apoptosis existed in buffalo antral follicles are confirmed by the above method.The GCs nuclear condensed and tended marginization in morphology,as well as apoptotic bodies was oberserved by TEM.Above results suggested that the GCs apoptosis of atretic antral follicles exists in buffalo and the GCs apoptosis are the potential mechanism of arosing antral follicles atresia in buffalo. |
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| Keywords: | buffalo apoptosis transmit electronic microscope follicular granulosa cells TUNEL |
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