| 侵染垂花悬铃花的木尔坦棉花曲叶病毒分子特征研究 |
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| 引用本文： | 汤亚飞,何自福,杜振国,韩利芳,佘小漫,罗方芳.侵染垂花悬铃花的木尔坦棉花曲叶病毒分子特征研究[J].植物病理学报,2013,43(2):120-127. |
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| 作者姓名： | 汤亚飞 何自福 杜振国 韩利芳 佘小漫 罗方芳 |
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| 作者单位： | 广东省农业科学院植物保护研究所 广东省植物保护新技术重点实验室,广州 510640 |
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| 基金项目： | 国家自然科学基金(31171817);国家国际科技合作项目（2011DFB30040）;广东省自然科学基金(9151065005000010、S2011040003309);广东省科技计划项目（2011B050400003） |
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| 摘 要： | 垂花悬铃花曲叶病是近期在广东发现的一种新病害,病株表现为叶片向上卷曲,叶脉肿大,叶脉变深绿色等症状。PCR检测结果显示, 该病样中均存在菜豆金色花叶病毒属病毒。基因克隆及序列分析结果表明,该病毒分离物（GD11）DNA-A全长为2 737 nt,具有菜豆金色花叶病毒属病毒基因组典型特征,为闭合环状单链DNA,编码6个ORFs;该序列与木尔坦棉花曲叶病毒(CLCuMV)各分离物序列的相似性均大于89.0%,其中与G6、Okra06及GX1等分离物序列的相似性大于99.0%。该病毒分离物也伴有卫星DNA β分子,其全长为1 348 nt,与CLCuMV各分离物的DNA β序列相似性大于85.0%,其中与G6、Okra06及GX1等DNA β的序列相似性均大于99.0%。因此,侵染广东垂花悬铃花的病毒分离物属于CLCuMV,且与入侵我国的朱槿分离物G6、黄秋葵分离物Okra06及棉花分离物GX1亲缘关系很近。本文首次报道了CLCuMV及其卫星β复合侵染垂花悬铃花。
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| 关 键 词： | 垂花悬铃花曲叶病 木尔坦棉花曲叶病毒 DNA-A 卫星DNA &beta |
| 收稿时间： | 2012-06-30; |
Molecular characterization of the Cotton leaf curl Multan virus infecting Malvaiscus arboreus |
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| TANG Ya-fei
,
HE Zi-fu
,
DU Zhen-guo
,
HAN Li-fang
,
SHE Xiao-man
,
LUO Fang-fang.Molecular characterization of the Cotton leaf curl Multan virus infecting Malvaiscus arboreus[J].Acta Phytopathologica Sinica,2013,43(2):120-127. |
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| Authors: | TANG Ya-fei HE Zi-fu DU Zhen-guo HAN Li-fang SHE Xiao-man LUO Fang-fang |
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| Institution: | Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China |
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| Abstract: | Malvaiscus arboreus leaf curl disease in Guangdong is a new disease. The diseased plants exhibits leaf curling upwards, vein swelling and vein dark green. The results of PCR detection indicated that the diseased plants were infected by a begomovirus. Virus isolate GD11 was obtained from M. arboreus leaf curl disease in Guangzhou, Guangdong Province. The complete nucleotide sequence of DNA-A was determined to be 2 737 nucleotides, encoding six potential ORFs. It was typical characteristics of Begomovirus. Comparisons showed that the DNA-A of GD11 had more than 89.0% sequence identity with all other isolates of Cotton leaf curl Multan virus (CLCuMV), and more than 99.0% sequence identity with isolates G6, Okra06 and GX1. The virus was also associated with satellite DNA β molecular,which contained 1 348 nucleotides. Pairwise comparison indicated that the DNA β of GD11 had more than 85.0% sequence identity with CLCuMV DNA β, and more than 99.0% sequence identify with isolates G6, Okra06 and GX1. These suggested that isolate GD11 infecting M. arboreus in Guangdong belonged to CLCuMV. Moreover it was most closely related to the isolate G6 infecting Hibiscus rosa-sinensis, the isolate Okra06 infecting Hibiscus esculentus in Guangdong and the isolate GX1 infecting Gossypium hirsutum in Guangxi. The paper is the first report on CLCuMV and its DNA β complex infecting M. arboreus. |
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| Keywords: | Malvaiscus arboreus leaf curl disease Malvaiscus arboreus leaf curl disease Cotton leaf curl Multan virus(CLCuMV Cotton leaf curl Multan virus(CLCuMV DNA-A &beta satellite DNA &beta |
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