| 李茎尖离体培养与植株再生 |
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| 引用本文： | 浦艳吉,李国怀,姚延兴,张陆阳,梁晓梅.李茎尖离体培养与植株再生[J].华中农业大学学报,2009,28(2). |
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| 作者姓名： | 浦艳吉 李国怀 姚延兴 张陆阳 梁晓梅 |
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| 作者单位： | 华中农业大学园艺植物生物学教育部重点实验室,武汉,430070 |
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| 基金项目： | 湖北省重点科技攻关项目 |
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| 摘 要： | 以欧洲李(Prunus domestica cv.'Tardicots')和樱桃李(Prunus cerasifera)试管苗为材料,建立了茎尖离体培养与植株再生体系.结果表明:欧洲李最适宜的培养基为MS+6-BA 0.5 mg/L+IBA 0.05 mg/L,0.1～0.2 mm的茎尖培养成苗率为64.16%,0.35～0.45 mm的茎尖培养成苗率为85.5%;樱桃李最适宜的培养基为LP+6-BA 0.5 mg/L+IBA 0.05 mg/L,0.1～0.2mm的茎尖培养成苗率为64.53%,0.35～0.45 mm的茎尖培养成苗率为84.7%.
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| 关 键 词： | 李 茎尖 离体培养 |
Shoot-tips in vitro Culture and Regeneration of Plum |
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| PU Yan-ji,LI Guo-huai,YAO Yan-xing,ZHANG Lu-yang,LIANG Xiao-mei.Shoot-tips in vitro Culture and Regeneration of Plum[J].Journal of Huazhong Agricultural University,2009,28(2). |
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| Authors: | PU Yan-ji LI Guo-huai YAO Yan-xing ZHANG Lu-yang LIANG Xiao-mei |
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| Institution: | Key Laboratory of Horticultural Plant Biology;Ministry of Education;Huazhong Agricultural University;Wuhan 430070;China |
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| Abstract: | Europe plum(Prunus domestica cv.'Tardicots') and myrobalan(P.cerasifera) were used as the materials and the regeneration system was established using the shoot-tips as explants.The results showed that the optimal medium for Europe plum shoot-tips culture was MS +6-BA 0.50 mg/L+IBA 0.05 mg/L,by 64.16% and 85.5% of seedling rate with 0.1~0.2 mm of shoot-tips and 0.35~0.45 mm of shoot-tips respectively.The optimal medium for myrobalan shoot-tips culture was LP +6-BA 0.5 mg/L+IBA 0.05 mg/L,by 64.53% and 84.7% o... |
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| Keywords: | plum shoot-tips in vitro culture |
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