| 海洋红酵母RH1菌株发酵培养条件的研究 |
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| 引用本文： | 杨莺莺,陈永青,杨铿,梁晓华,徐创文,梁润捷,洪敏娜.海洋红酵母RH1菌株发酵培养条件的研究[J].南方水产,2012,8(1):67-74. |
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| 作者姓名： | 杨莺莺 陈永青 杨铿 梁晓华 徐创文 梁润捷 洪敏娜 |
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| 作者单位： | 中国水产科学研究院南海水产研究所,广东广州,510300 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目，现代农业(虾)产业技术体系建设专项资金，公益性行业(农业)科研专项经费项目，广东省海洋渔业科技推广专项，广东省鱼病防治专项，科技人员服务企业行动项目 |
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| 摘 要： | 该研究比较了不同碳源、氮源、无机盐对海洋红酵母菌（Rhodotorula sp.）RH1菌株发酵产量的影响,通过葡萄糖、蛋白胨、酵母膏和硫酸镁（MgSO4）4因素3水平的正交试验,确定了RH1菌株的最优培养基为蛋白胨10 g.L-1,酵母膏15 g.L-1,葡萄糖20 g.L-1,MgSO40.25 g.L-1,磷酸二氢钾（KH2PO4）0.25 g.L-1,氯化钠（NaCl）10 g.L-1。结果显示,该菌株最佳摇瓶发酵条件为接种量10%,初始pH 5.0,摇瓶装液量80 mL/500 mL三角瓶,培养温度28℃,经48 h培养,菌量可达10.46×108cfu.mL-1,比优化条件前提高23.9%。还进行了RH1菌株25 L发酵罐扩大培养试验,在接种量为8%、初始pH 5.0、搅拌速率350～400 r.min-1、通气量9 L.min-1、温度28℃的条件下,经28 h的培养,菌量可达33.6×108cfu.mL-1。预计通过连续补料的方式进行培养,有望进一步提高菌量。
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| 关 键 词： | 海洋红酵母 培养条件 优化 |
Study on fermentation conditions for oceanic red yeast strain RH1 |
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| YANG Yingying
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CHENG Yongqing
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YANG Keng
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LIANG Xiaohua
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XU Chuangwen
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LIANG Runjie
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HONG Minna.Study on fermentation conditions for oceanic red yeast strain RH1[J].South China Fisheries Science,2012,8(1):67-74. |
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| Authors: | YANG Yingying CHENG Yongqing YANG Keng LIANG Xiaohua XU Chuangwen LIANG Runjie HONG Minna |
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| Institution: | (South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China) |
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| Abstract: | We compared the effects of different carbon sources,nitrogen sources and inorganic salts on fermentation yield of oceanic red yeast（Rhodotorula sp.）RH1.By 4 factors and 3 levels orthogonal test,the optimum fermentation conditions for RH1 were determined： peptone 10 g·L-1,yeast extract 15 g·L-1,glucose 20 g·L-1,MgSO4 0.25 g·L-1,KH2PO4 0.25 g·L-1,NaCl 10 g·L-1,inoculation amount 10%,initial pH 5.0,temperature 28 ℃,80 mL medium in 500 mL triangle bottle,and fermentation time 48 h.Under above conditions,the cell biomass of RH1 can reach 10.46×108 cfu·mL-1,which increases by 23.9% as compared with the fermentation before optimization.An experiment of expanded culture is also conducted in a 25 L fermentation tank.The cell biomass of RH1 increased to 33.6×108 cfu·mL-1 in 28 h under the conditions of inoculation amount 8%,initial pH 5.0,revolution speed 350~400 r·min-1,air ventilation volume 9 L·min-1 and temperature 28 ℃.It is expected to further increase the biomass of strain RH1 by continuous filling of the materials. |
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| Keywords: | Rhodotorula sp culture conditions optimization |
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