| 生长点点滴法在筛选鉴定转基因植株中的应用 |
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| 引用本文： | 金万梅,巩振辉,吕元红.生长点点滴法在筛选鉴定转基因植株中的应用[J].西北农林科技大学学报(社会科学版),2002,30(5):23-26. |
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| 作者姓名： | 金万梅 巩振辉 吕元红 |
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| 作者单位： | 西北农林科技大学,园艺学院,陕西,杨陵,712100 |
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| 基金项目： | 国家自然科学基金项目 (3 9770 5 2 2 )，陕西省自然科学基金项目 (2 0 0 1SM11)，科技部杨凌生物育种中心基金项目 (99-18) |
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| 摘 要： | 对转化体 (种子或幼苗 )筛选的培养基筛选法和生长点点滴筛选法进行了比较分析。结果表明 ,在 4d苗龄的转化芥菜幼苗生长点点滴 80 0 mg/L 的潮霉素 ,获得了转基因植株 ,PCR检测结果表明 ,两种筛选方法的筛选结果一致。生长点点滴法的优点是 ,转基因幼苗不易污染 ,且成活率高。
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| 关 键 词： | 芥菜 转基因植株 筛选方法 生长点点滴法 |
| 文章编号： | 1671-9387(2002)05-0023-04 |
| 收稿时间： | 2002/9/13 0:00:00 |
| 修稿时间： | 2002年9月13日 |
Dripping antibiotic solution on growing point as a novel approach to screening transgenic plants |
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| JIN Wan mei,GONG Zhen hui,LU Yuan hong.Dripping antibiotic solution on growing point as a novel approach to screening transgenic plants[J].Journal of Northwest Sci-Tech Univ of Agr and,2002,30(5):23-26. |
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| Authors: | JIN Wan mei GONG Zhen hui LU Yuan hong |
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| Institution: | JIN Wan mei,GONG Zhen hui,LU Yuan hong (College of Horticulture,Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China) |
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| Abstract: | The two methods of screening transgenic plants,including transgenic seeds sowed in the medium with hygromycin (TSSM) and dripped hygromycin solution on the growing point of transgenic seedling (DSGPTS),were compared and analysed.The results indicated that the transgenic plants were obtained with the method of DSGPTS,when 800 mg/L of hygromycin was dripped on the growing point of transgenic seedling at its age of 4 days.PCR analysis showed that the results of both TSSM method and DSGPTS method were showing no difference.The advantage of DSGPTS method is that the transgenic seedling is safe from pollution and the percentage of survival for the transgenic seedling is high. |
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| Keywords: | Brassica juncea transgenic plants screening method method of dripping antibiotic solution on growing point |
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