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| 不同培养条件对大花金挖耳愈伤组织生长及黄酮产量的影响 | |
| 引用本文： | 李玉平,姜在民,冯俊涛,徐珊,张兴.不同培养条件对大花金挖耳愈伤组织生长及黄酮产量的影响[J].核农学报,2008,22(3):286-291. |
| 作者姓名： | 李玉平 姜在民 冯俊涛 徐珊 张兴 |
| 作者单位： | 1. 西北农林科技大学,无公害农药研究服务中心,陕西,杨陵,712100;西北农林科技大学生命科学学院,陕西,杨陵,712100 2. 西北农林科技大学生命科学学院,陕西,杨陵,712100 3. 西北农林科技大学,无公害农药研究服务中心,陕西,杨陵,712100 |
| 摘 要： | 以大花金挖耳愈伤组织为材料,通过测定愈伤组织生长量、黄酮含量和产量等指标,研究了不同培养条件对大花金挖耳愈伤组织生长及其黄酮产量的影响,为大规模细胞培养生产大花金挖耳药用成分黄酮奠定基础。结果表明:不同外植体愈伤组织的黄酮含量为根>子叶>下胚轴,黄酮产量以根愈伤组织为最高;继代次数在3~12代愈伤组织的黄酮产量较为稳定;愈伤组织生长及黄酮生物合成的适宜培养温度为25℃;10min紫外线照射引起黄酮含量的提高;MS、B5、NT及其组合培养基中,大花金挖耳愈伤组织生长及黄酮产量随培养时间的延长,均表现出先升高后降低的趋势,在接种35-40d后愈伤组织黄酮产量达到最高值,其中NT培养基中黄酮产量高于其他培养基中愈伤组织黄酮产量。 |
| 关 键 词： | 大花金挖耳 愈伤组织 培养条件 黄酮产量 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
EFFECTS OF DIFFERENT CULTURAL CONDITIONS ON CALLUS GROWTH AND FLAVONOID YIELD OF Carpesum.macrocephalum |
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| LI Yu-ping,JIANG Zai-min,FENG Jun-tao,XU Shan,ZHANG Xing.EFFECTS OF DIFFERENT CULTURAL CONDITIONS ON CALLUS GROWTH AND FLAVONOID YIELD OF Carpesum.macrocephalum[J].Acta Agriculturae Nucleatae Sinica,2008,22(3):286-291. | |
| Authors: | LI Yu-ping JIANG Zai-min FENG Jun-tao XU Shan ZHANG Xing |
| Abstract: | The objective of the current study was to set a foundation for efficient large scale cultivation for biosynthesis of flavonoids.The effect of several factors on callus growth amount,flavonoid content and yield in callus cultures of Carpesium.macrocephalum Franch.et Sav were investigated.The results showed that: ① the flavonoid content and yield of root-induced callus was higher than hypocotyl and cotyledon-induced callus;② the flavonoid yield of root-induced callus was stable during 3～12 subculture generation;③ the optimum temperature for callus growth and flavonoid cumulation was 25℃;④ the optimum ultraviolet radiation time for callus flavonoid content was 10 min;⑤ the callus growth and flavonoid yield increased first and then decreased at different culture medium,the flavonoid yield reached the maximum after 35～40 days of inoculation,and the optimal culture medium to produce flavonoid was NT. |
| Keywords: | Carpesium macrocephalum callus cultural condition flavonoid yield |
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