| 石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响 |
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| 引用本文： | 王金成,井明博,段春燕,王国锋,石国玺,周天林.石油烃污染对陇东黄土高原土壤生物学及非生物学特性的影响[J].水土保持通报,2017,37(1):9-16. |
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| 作者姓名： | 王金成 井明博 段春燕 王国锋 石国玺 周天林 |
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| 作者单位： | 1. 甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃 庆阳 745000;陇东学院生命科学与技术学院,甘肃 庆阳 745000;2. 陇东学院生命科学与技术学院,甘肃 庆阳,745000;3. 陕西师范大学 生命科学学院,陕西 西安,710062;4. 甘肃省高校陇东生物资源保护与利用省级重点实验室,甘肃 庆阳 745000;陇东学院生命科学与技术学院,甘肃 庆阳 745000;中国科学院 西北高原生物研究所,青海 西宁 810008 |
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| 基金项目： | 甘肃省科技支撑计划项目“陇东黄土高原地区石油污染土壤原位修复技术研究与示范”（1204FKCM173）；甘肃省教育厅科技计划项目“陇东黄土高原石油污染土壤微生物群落动态分析及石油降解菌的筛选”（2014B-091）；国家自然科学资助项目（31500427）；甘肃省庆阳市科技支撑计划项目（GC2011-16）；陇东学院青年科技创新项目（XYZK1603） |
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| 摘 要： | 目的]探索石油污染与土壤生物学及非生物学特性间相互关系,为陇东黄土高原地区石油污染土壤的综合治理提供基础数据和背景资料。方法]采用常规方法测定甘肃省庆阳市5县,1区,8个采油区油污土壤化性质、酶活性和土壤微生物遗传多样性。结果]土壤总石油烃含量(TPHs)与碱解氮、速效磷、pH值和脲酶呈负相关(p0.01),与土壤多酚氧化酶、脱氢酶和过氧化氢酶呈正相关(p0.01);样地土壤TPHs含量低的土壤微生物Shannon-Wiener指数明显高于TPHs含量高的样地;NMDS排序结果显示,石油污染对该地区土壤影响最大的因子为土壤碱解氮和速效磷,其次为TPHs,pH值、脲酶、ShannonWiener指数及过氧化氢酶。结论]陇东地区石油烃污染对土壤环境因子的影响主要表现在包括氮、磷等营养元素的有效循环、土壤脲酶活性和微生物群落多样性,而对土壤微生物群落丰富度、均匀度和速效钾的影响未达到显著水平。
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| 关 键 词： | 陇东黄土高原 石油烃污染 土壤理化因子 微生物群落 |
| 收稿时间： | 2016/6/21 0:00:00 |
| 修稿时间： | 2016/9/20 0:00:00 |
Impact of Crude Oil Pollution on Soil Biological and Abiological Properties in Eastern Gansu Province |
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| WANG Jincheng,JING Mingbo,DUAN Chunyan,WANG Guofeng,SHI Guoxi and ZHOU Tianlin.Impact of Crude Oil Pollution on Soil Biological and Abiological Properties in Eastern Gansu Province[J].Bulletin of Soil and Water Conservation,2017,37(1):9-16. |
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| Authors: | WANG Jincheng JING Mingbo DUAN Chunyan WANG Guofeng SHI Guoxi and ZHOU Tianlin |
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| Institution: | Provincial Key University Laboratory for Protection and Utilization of Longdong Bioresources in Gansu Province, Qingyang, Gansu 745000, China;College of Life Science and Technology, Longdong University, Qingyang, Gansu 745000, China,Provincial Key University Laboratory for Protection and Utilization of Longdong Bioresources in Gansu Province, Qingyang, Gansu 745000, China;College of Life Science and Technology, Longdong University, Qingyang, Gansu 745000, China,College of Life Science and Technology, Longdong University, Qingyang, Gansu 745000, China,College of Life Science, Shaanxi Normal University, Xi''an, Shaanxi 710062, China,Provincial Key University Laboratory for Protection and Utilization of Longdong Bioresources in Gansu Province, Qingyang, Gansu 745000, China;College of Life Science and Technology, Longdong University, Qingyang, Gansu 745000, China;Chinese Academy of Sciences, Northwest Institute of Plateau Biology, Xining, Qinghai 810008, China and Provincial Key University Laboratory for Protection and Utilization of Longdong Bioresources in Gansu Province, Qingyang, Gansu 745000, China;College of Life Science and Technology, Longdong University, Qingyang, Gansu 745000, China |
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| Abstract: | Objective] The objective of this study is to explain relationship between soil biology and abiological characteristics on crude-oil contaminated soil and provide basic data and background information for its comprehensive remediation in eastern Gansu Province. Methods] The changes of soil physical and chemical properties as well as enzyme activities in crude oil-contaminated soil in Qingyang City of Gansu Province were investigated using the conventional methods, and the genetic diversity of microbial community was analyzed based on PCR-DGGE. Results] The contents of soil available N, available P, pH value and activity of soil urease decreased with the increase of soil TPHs concentration respectively(p<0.01), while the activities of soil polyphenoloxidase, dehydrogenase and catalase had a significant positive correlation with soil TPHs concentration respectively(p<0.01). As for the genetic diversity of soil microbial communities, Shannon-Wiener index in the studied sites with lower TPHs concentration was significantly higher than that of the sites with higher TPHs concentration(p<0.05). Moreover, nonmetric multidimensional scaling(NMDS) showed that petroleum-contaminated soil in Gansu Province were strongly affected by soil available N and available P, and following-affected by soil TPHs concentration, pH value, urease activity, Shannon-Wiener index of soil microbial communities and activity of soil catalase.Conclusion] Impact of crude oil pollution on soil environmental factors in Eastern Gansu Province of Loess Plateau was mainly reflected by the nutrients effective circulation of nitrogen and phosphorus as well as soil microbial-communities diversity and soil urease activity, but there were no significant impact on available K, richness and evenness of soil microbial communities. |
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| Keywords: | Eastern Gansu Province of Loess Plateau crude oil pollution soil environmental factors microbial community |
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