| 甘蔗线条花叶病毒HC-Pro基因的分子变异分析 |
| |
| 引用本文： | 贺 振,李文凤,张志想,李世访.甘蔗线条花叶病毒HC-Pro基因的分子变异分析[J].植物保护,2017,43(2):29-36. |
| |
| 作者姓名： | 贺 振 李文凤 张志想 李世访 |
| |
| 作者单位： | 1. 扬州大学园艺与植物保护学院,扬州225009;中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京 100193;2. 云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室,开远,661699;3. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京,100193 |
| |
| 基金项目： | 植物病虫害生物学国家重点实验室开放课题(SKLOF201518); 扬州大学科技创新培育基金(2015CXJ043) |
| |
| 摘 要： | 为了解析甘蔗线条花叶病毒Sugarcane streak mosaic virus(SCSMV)不同分离物HC-Pro基因的分子变异规律,本研究利用RT-PCR法扩增获得SCSMV HC-Pro基因的序列,通过生物信息学分析,分别从重组、系统发生、选择压力等方面研究SCSMV HC-Pro基因的分子变异特征。共测定了44条SCSMV HC-Pro基因序列,相似性最低值为70%;HC-Pro基因重组频率较低,仅发现3个重组位点,其中一个系首次报道;与先前报道相比,部分新测定云南蔗区的SCSMV分离物在HC-Pro基因上形成一个新组-第Ⅲ组;HC-Pro基因处于很强的负选择压力作用,未发现正向选择作用位点。本研究结果进一步证明SCSMV HC-Pro基因具有高度的遗传多样性。
|
| 关 键 词： | 甘蔗线条花叶病毒 HC-Pro基因 分子变异 |
| 收稿时间： | 2016/3/30 0:00:00 |
| 修稿时间： | 2016/4/23 0:00:00 |
Molecular variation of HC-Pro gene of Sugarcane streak mosaic virus |
| |
| He Zhen,Li Wenfeng,Zhang Zhixiang,Li Shifang.Molecular variation of HC-Pro gene of Sugarcane streak mosaic virus[J].Plant Protection,2017,43(2):29-36. |
| |
| Authors: | He Zhen Li Wenfeng Zhang Zhixiang Li Shifang |
| |
| Institution: | 1. School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3. Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661600, China |
| |
| Abstract: | The objective of this study is to assess the molecular evolution and divergence of SCSMV according to HC-Pro gene sequences.The HC-Pro gene sequences of SCSMV were obtained by RT-PCR,and analyzed by bioinformatic software,in aspect of recombination,phylogenetics,selection,demography,and gene flow.In the present study,44 HC-Pro gene sequences were determined with a 70％ lowest similarity;only one novel recombination site together with two previous reported sites were found in HC-Pro gene,suggesting that infrequent recombination events were distributed in this gene of SCSMV;one novel lineage (lineage Ⅲ) clustered by SCSMV sequences determined here was found;strong purifying selection was found in the HC-Pro gene of SCSMV.Our genetic study further indicates that the HC-Pro gene showed high genetic diversity in the genome of SCSMV. |
| |
| Keywords: | Sugarcane streak mosaic virus HC-Pro molecular variation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《植物保护》浏览原始摘要信息 |
| 点击此处可从《植物保护》下载免费的PDF全文 |
|
