| 木质素对灵芝菌丝体生长的影响 |
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| 引用本文： | 裴海生,孙君社,王民敬,尹腾,张秀清.木质素对灵芝菌丝体生长的影响[J].农业工程学报,2017,33(6):309-314. |
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| 作者姓名： | 裴海生 孙君社 王民敬 尹腾 张秀清 |
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| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京 100083;农业部规划设计研究院,北京 100125;2. 农业部规划设计研究院,北京,100125;3. 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 基金项目： | 国家自然科学基金（21576142） |
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| 摘 要： | 为了解添加外源木质素对灵芝菌丝体生长的影响,该研究通过在灵芝培养基中添加纤维素、半纤维素和木质素,考察了外源添加木质纤维素主要组分对灵芝菌体生长的影响,发现木质素添加明显促进灵芝菌丝体生长;进一步考察添加不同浓度木质素灵芝菌丝体生长情况以及灵芝产多糖、三萜变化规律。结果表明少量添加木质素对灵芝菌丝体生长有明显促进作用,木质素加量为1.0%时,灵芝菌丝体生长情况最好,生物量比对照提高了56%,同时灵芝胞外多糖、胞内多糖及灵芝胞外三萜、胞内三萜的合成均有明显提高,其中胞内多糖提高43%;4种灵芝菌株发酵产多糖、三萜结果对比分析表明1.0%木质素添加均能提高不同菌株发酵多糖、三萜含量,木质素作为营养基质在不同灵芝菌株培养过程中添加均能促进菌丝体活性物质合成。研究结果可为灵芝菌生长代谢研究及人工栽培提供科学依据和参考。
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| 关 键 词： | 木质素 多糖 生物量 三萜 灵芝菌 液体发酵 |
| 收稿时间： | 2017/1/4 0:00:00 |
| 修稿时间： | 2017/3/16 0:00:00 |
Effect of lignin on growth of Ganoderma lucidum |
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| Pei Haisheng,Sun Junshe,Wang Minjing,Yin Teng and Zhang Xiuqing.Effect of lignin on growth of Ganoderma lucidum[J].Transactions of the Chinese Society of Agricultural Engineering,2017,33(6):309-314. |
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| Authors: | Pei Haisheng Sun Junshe Wang Minjing Yin Teng and Zhang Xiuqing |
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| Institution: | 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. Chinese Academy of Agricultural Engineering, Beijing 100125, China;,2. Chinese Academy of Agricultural Engineering, Beijing 100125, China;,2. Chinese Academy of Agricultural Engineering, Beijing 100125, China;,1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; and 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; |
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| Abstract: | Abstract: Ganoderma lucidum is a typical polypore fungus used for traditional Chinese medical purposes. The active ingredients of Ganoderma lucidum include polysaccharides, triterpene, proteins and ganoderic acid, which can be effective in the treatment of cancer, hypertension and viral infections. Therefore, it has been widely planted and used in human health products. Nowadays the main cultivation methods for Ganoderma lucidum were cut-log cultivation and substitute cultivation. These two methods use abundant wood. So the cultivation of Ganoderma lucidum by using abundant wood could cause destruction to the ecological environment. Ganoderma lucidum liquid fermentation cultivation was no only an important method for fundamental research, but also a way to product bioactive compounds. In this study, cellulose, hemicellulose and lignin were added into the media to investigate the influence of these additives on the growth of Ganoderma lucidum. The results showed that adding lignin or hemicellulose all could obviously promote the growth of Ganoderma lucidum. However, adding cellulose could not obviously promote the growth of Ganoderma lucidum. Most of lignin and a part of hemicellulose could be used, while the cellulose could not be used for the growth of Ganoderma lucidum. The effects of liquid culture media with different lignin concentrations on the growth of Ganoderma lucidum were studied. The liquid fermentation media with four different lignin concentrations (0.2%, 0.5%, 1.0%, 1.5%) were designed. The results showed that adding little lignin could promote the growth of Ganoderma lucidum, however, when the concentration of lignin was above 1.0%, the growth of Ganoderma lucidum was severely inhibited. Compared with the control, the growth of Ganoderma lucidum was the best with the lignin concentration of 1%, and its biomass was increased by 56%; the synthesis of extracellular polysaccharides and intracellular polysaccharides were increased by 33.6%, 43%, respectively; and the synthesis of extracellular triterpenoid and intracellular triterpenoid were increased by 34.6%, 41%, respectively. It was proved that adding lignin in the process of liquid fermentation culture could increase the yield of polysaccharides and triterpenoid in four different Ganoderma lucidum strains (Ganoderma lucidum 203, wild Ganoderma lucidum, Long Quan Ganoderma lucidum, Jin Zhai Ganoderma lucidum. Compared with the control, the polysaccharide contents of four Ganoderma lucidum were increased by 38%-43%, and triterpenoid contents were increased by 30%-38%, and the polysaccharide content of Ganoderma lucidum 203 was higher than the control by 43%, and triterpenoid content of Jin Zhai Ganoderma lucidum was higher than the control by 38%. However, it will need further study to prove that adding lignin between cut-log cultivation or substitute cultivation can promote the growth of Ganoderma lucidum and the synthesis of bioactive compounds. |
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| Keywords: | lignin polysaccharides triterpenes Ganoderma lucidum liquid fermentation |
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