| 犬巴贝西虫病PCR检测方法的建立 |
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| 引用本文： | 尚泽松,李小康,王雪莹,张才,江丰伟,赵艳辉.犬巴贝西虫病PCR检测方法的建立[J].河南农业科学,2012,41(5):158-160. |
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| 作者姓名： | 尚泽松 李小康 王雪莹 张才 江丰伟 赵艳辉 |
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| 作者单位： | 河南科技大学动物科技学院,河南洛阳,471003 |
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| 基金项目： | 河南科技大学SRTP项目 |
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| 摘 要： | 为快速、准确检测犬巴贝西虫病,根据GenBank中收录的犬的巴贝西虫18sRNA基因序列,设计合成1对特异性诊断引物,建立了PCR诊断方法。结果扩增出了犬巴贝西虫18sRNA基因的一段大小为319bp片段,经测序,所扩增序列与报道的序列完全匹配。同时对附红细胞体、巴氏杆菌DNA样品进行扩增没有出现扩增条带。阳性犬全血DNA稀释1 000倍后,依然可以有效地检测出虫体DNA。结果表明,所建立的PCR检测方法,具有极高的敏感性和特异性,可以运用于临床检测巴贝西虫病。
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| 关 键 词： | PCR 犬 巴贝西虫 18sRNA基因 |
Establishment of A PCR Assay for Detecting Canine Babesia |
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| SHANG Ze-song
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LI Xiao-kang
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WANG Xue-ying
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ZHANG Cai
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JIANG Feng-wei
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ZHAO Yan-hui.Establishment of A PCR Assay for Detecting Canine Babesia[J].Journal of Henan Agricultural Sciences,2012,41(5):158-160. |
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| Authors: | SHANG Ze-song LI Xiao-kang WANG Xue-ying ZHANG Cai JIANG Feng-wei ZHAO Yan-hui |
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| Institution: | (College of Animal Science and Technology,Henan University of Science and Technology, Luoyang 471003,China) |
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| Abstract: | One pair of specific primers was designed according to the 18sRNA gene sequence of canine Babesia,and then a PCR assay was established for detecting canine Babesia.A fragment in length of 319 bp was amplified by PCR and the sequence homology to those from GenBank was 100%.No PCR products were amplified from purified DNA of Eperythrozoon or Pasteurella.This method can detect the genomic DNA of Canine Babesia in 10-3 diluted whole blood DNA of infected dogs.This indicates that the PCR method was precise,sensitive and specific. |
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| Keywords: | PCR dog Babesia 18sRNA gene |
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