An inter-laboratory comparison of multi-enzyme and multiple substrate-induced respiration assays to assess method consistency in soil monitoring |
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| Authors: | Rachel E Creamer Pat Bellamy Helaina I J Black Clare M Cameron Colin D Campbell Paul Chamberlain Jim Harris Nisha Parekh Mark Pawlett Jan Poskitt Dote Stone Karl Ritz |
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| Institution: | (1) Natural Resources Department, School of Applied Sciences, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK;(2) The Macaulay Institute, Craigiebuckler, Aberdeen, AB15 8QH, UK;(3) CEH Lancaster, Lancaster Environment Centre, Bailrigg, Lancaster, LA1 4AP, UK;(4) Teagasc, Johnstown Castle Environment Centre, County Wexford, Ireland;(5) Department Soil and Environment, Swedish University Agricultural Sciences, Uppsala, Sweden |
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| Abstract: | The use of indicators in soil monitoring schemes to detect changes in soil quality is receiving increased attention, particularly
the application of soil biological methods. However, to date, the ability to compare information from different laboratories
applying soil microbiological techniques in broad-scale monitoring has rarely been taken into account. This study aimed to
assess the consistency and repeatability of two techniques that are being evaluated for use as microbiological indicators
of soil quality: multi-enzyme activity assay and multiple substrate-induced respiration (MSIR). Data were tested for intrinsic
(within-assay plate) variation, inter-laboratory repeatability (geometric mean regression and correlation coefficient) and
land-use discrimination (principal components analysis). Intrinsic variation was large for both assays suggesting that high
replicate numbers are required. Inter-laboratory repeatability showed diverging patterns for the enzyme assay and MSIR. Discrimination
of soils was significant for both techniques with relatively consistent patterns; however, combined laboratory discrimination
analyses for each technique showed inconsistent correspondence between the laboratories. These issues could be addressed through
the adoption of reliable analytical standards for biological methods along with adequate replication. However, until the former
is addressed, dispersed analyses are not currently advisable for monitoring schemes. |
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| Keywords: | CLPP Multiple substrate-induced respiration Repeatability Soil enzymes Soil monitoring |
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