| DNA条形码基因ITS·ITS2及rbcL在苍耳属可采用相同的PCR条件（英文） |
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| 引用本文： | 胡伟毅,汪连军,盛志超.DNA条形码基因ITS·ITS2及rbcL在苍耳属可采用相同的PCR条件（英文）[J].农业科学与技术,2013(9):1212-1214. |
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| 作者姓名： | 胡伟毅 汪连军 盛志超 |
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| 作者单位： | 连云港出入境检验检疫局,江苏连云港222042 |
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| 基金项目： | Supported by Science and Technology Project of Jiangsu Entry-Exit Inspection and Quarantine Bureau（2012KJ54）~~ |
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| 摘 要： | 目的]为植物DNA条形码标准基因筛选研究提供参考,减少植物DNA条形码研究中的工作量。方法]对7种苍耳属植物ITS、ITS2及rbcL基因采用相同的扩增条件（95℃4 min;35 cycles：94℃30 s;52℃45 s;72℃45 s];72℃10 min;4℃保存）。结果]3种DNA条形码基因同时成功扩增。结论]这说明植物DNA条形码基因中ITS、ITS2及rbcL的PCR条件存在合并可能性。
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| 关 键 词： | DNA条形码 苍耳属 ITS ITS2 rbcL |
PCR Amplification System of DNA Barcoding Genes ITS,ITS2 and rbcL from Xanthium |
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| Weiyi HU;Lianjun WANG;Zhichao SHENG.PCR Amplification System of DNA Barcoding Genes ITS,ITS2 and rbcL from Xanthium[J].Agricultural Science ＆ Technology,2013(9):1212-1214. |
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| Authors: | Weiyi HU;Lianjun WANG;Zhichao SHENG |
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| Institution: | Weiyi HU;Lianjun WANG;Zhichao SHENG;Lianyungang Entry-exit Inspection and Quarantine Bureau; |
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| Abstract: | Objective] This study aimed to provide reference and reduce the workload for screening standard DNA barcoding genes of plants. Method] Three DNA barcoding genes ITS, ITS2 and rbcL were amplified from seven Xanthium species under the same PCR condition： PCR amplification was started with initial denaturation at 95 ℃ for 4 min, followed by 35 cycles of denaturation at 94 ℃ for 30 s, annealing at 52 ℃ for 45 s, and extension at 72 ℃ for 45 s; the amplification was completed by holding the reaction mixture at 72 ℃ for 10 min to allow complete extension of PCR, and the PCR products were stored at 4 ℃. Result] Three DNA barcoding genes ITS, ITS2 and rbcL were all amplified successfully. Conclusion] This study indicates that PCR amplification conditions for DNA barcoding genes ITS,ITS2 and rbcL in plants may be consistent. |
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| Keywords: | DNA barcoding Xanthium ITS ITS2 rbcL |
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