| 鸡蛋清中3种蛋白质的连续化提取工艺初步研究 |
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| 引用本文： | 傅冰,俞汇颖,魏云林,季秀玲,郑维桃.鸡蛋清中3种蛋白质的连续化提取工艺初步研究[J].农业科学与技术,2013(11):1651-1654,1692. |
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| 作者姓名： | 傅冰 俞汇颖 魏云林 季秀玲 郑维桃 |
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| 作者单位： | [1]丽水职业技术学院环境工程学院,浙江丽水323000 [2]昆明理工大学生命科学与技术学院,云南昆明650500 [3]丽水绿谷食品有限公司,浙江丽水323000 |
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| 基金项目： | 丽水市科技局校企项目（20080410）. |
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| 摘 要： | 目的]研究鸡蛋清中溶菌酶、卵转铁蛋白和卵白蛋白的分离提取工艺。方法]采用硫酸铵沉淀和离子交换层析分离三种蛋白质，并用聚丙烯酰胺凝胶电泳（SDS-PAGE）检测其纯度。结果]层析纯化后 SDS-PAGE检测其纯度达到电泳纯，溶菌酶的比活由144.13 U/mg提高到2235 U/mg，纯化倍数达15倍，回收率为15.76%；卵转铁蛋白抑菌率为48.84%。结论]该方法分离溶菌酶、卵转铁蛋白和卵白蛋白，工艺路线简单、快捷、成本低，适用于工业生产。
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| 关 键 词： | 鸡蛋清 溶菌酶 卵白蛋白 卵转铁蛋白 离子交换层析 分离 |
Preliminary Study on the Separation of Lysozyme,Ovotransferrin and Ovalbumin from Egg White |
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| Bing FU,Huiying YU,Yunlin WEI,Xiuling JI,Weitao ZHENG.Preliminary Study on the Separation of Lysozyme,Ovotransferrin and Ovalbumin from Egg White[J].Agricultural Science ＆ Technology,2013(11):1651-1654,1692. |
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| Authors: | Bing FU Huiying YU Yunlin WEI Xiuling JI Weitao ZHENG |
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| Institution: | 1. Branch of Environment Engineering, Lishui Vocational ＆ Technical College Lishui, 323000, China; 2. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China; 3. Lishui Green Vally Food Co. Ltd, Lishui 323000, China |
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| Abstract: | Objective] A study on separation process of lysozyme, ovotransferrin and ovalbumin from egg white. Method] The proteins were separated by ammonium sul-fates and ion-exchange chromatography. Purity of the proteins was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. Result] The results showed that the proteins were electrophoresis-pure. The specific activity of lysozyme was increased from 144.13 to 2 235 U/mg, and purification factor was 15-fold. Lysozyme recovery rate was estimated to be 15.76%. Bacteriostasis rate of ovotransferrin was 48.84%. Conclusion] The procedure for separating lysozyme, ovotransferrin and ovalbumin from egg white was simple, fast, low-cost and suitable for industrilization. |
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| Keywords: | Egg white Llysozyme Ovalbumin Ovotransferrin Ionexchange chro-matography Separation |
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