| 不同生长素对绣球丁香扦插生根的影响及其生根过程中相关酶活性的变化（英文） |
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| 引用本文： | 颜婷美,张安琪,王峰,李承秀,于永畅,牛田,王郑昊,王长宪.不同生长素对绣球丁香扦插生根的影响及其生根过程中相关酶活性的变化（英文）[J].农业科学与技术,2013(12):1714-1718. |
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| 作者姓名： | 颜婷美 张安琪 王峰 李承秀 于永畅 牛田 王郑昊 王长宪 |
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| 作者单位： | [1]山东农业大学林学院,山东泰安271018 [2]泰山林业科学研究院,山东泰安271000 [3]泰安市徂徕山林场,山东泰安271000 |
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| 摘 要： | 目的]研究不同生长素对绣球丁香扦插生根的影响及生根过程中相关酶活性的变化。方法]以绣球丁香为材料,选用IBA、ABT、NAA 3种生长素分别于500、1 000、1 500、2 000 mg/L浓度下进行嫩枝扦插对比试验,研究生根过程中过氧化物酶（POD）、多酚氧化酶（PPO）和吲哚乙酸氧化酶（IAAO）的活性变化。结果]IBA、ABT、NAA 3种生长素的最适浓度分别为1 500、1 000、1 000 mg/L,其中以IBA 1 500 mg/L处理效果最佳。在整个生根过程中,处理组与对照组POD与PPO活性动态变化趋势基本一致,但处理组的POD与PPO活性显著提高且变化幅度更大;扦插初期对照组与处理组的IAAO活性呈相反的变化趋势,前者缓慢升高而后者迅速下降,且处理组显著低于对照组;此外,较高活性的POD与PPO利于不定根的诱导,较低活性的IAAO则利于不定根的发育与伸长。结论]该研究初步揭示了绣球丁香嫩枝扦插的生根机理。
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| 关 键 词： | 绣球丁香 生长素 扦插生根 酶活性 |
Effects of Different Exogenous Hormones on Rooting of Syringa microphylla Cuttings and Change in Related Enzyme Activity During the Rooting Process |
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| Tingmei YAW,Anqi ZHANG,Feng WANG,Chengxiu LI,Yongchang YU,Tian NIU,Zhenghao WANG,Changxian WANG.Effects of Different Exogenous Hormones on Rooting of Syringa microphylla Cuttings and Change in Related Enzyme Activity During the Rooting Process[J].Agricultural Science ＆ Technology,2013(12):1714-1718. |
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| Authors: | Tingmei YAW Anqi ZHANG Feng WANG Chengxiu LI Yongchang YU Tian NIU Zhenghao WANG Changxian WANG |
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| Institution: | 1. College of Forestry, Shandong Agricultural University, Tai＇an 271018, China; 2. Taishan Academy of Forestry, Tai＇an 271018, China; 3. Culaishan Tree Tarm of Tai＇an City, Tai＇an 271018, China |
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| Abstract: | Objective] This study aimed to investigate the effects of different exoge- nous hormones on the rooting of Syringa microphylla cuttings and the change in related enzymes activity during the rooting process. Method] Three different exoge- nous hormones IBA, NAA and ABT, each with concentrations of 500, 1 000, 1 500 and 2 000 mg/L were used to treat S. microphylla cuttings, and changes in the ac- tivities of peroxidase （POD）, poiyphenol oxidase （PPO） and indoleacetic acid oxidase （IAAO） during the rooting process were also investigated. Result] The most appro- priate concentrations of IBA, ABT and NAA were 1 500, 1 000 and 1 000 mg/L, respectively, and the 1 500 mg/L IBA treatment exhibited the best effect on rooting. Throughout the rooting process, POD and PPO activities showed the same trends in the treatment groups as those in the control group, but the POD and PPO activi- ties in the treatment groups were increased significantly, with greater amplitude of variation; at the early stage, IAAO activity exhibited an opposite trend between the control group and the treatment groups, which increased slowly in the former, but decreased rapidly in the latter, and it was significantly lower in the treatment groups compared to the control; additionally, higher POD and IAAO activities were con- ducive to the induction of adventitious roots, and lower POD and IAAO activities fa- vored their formation and elongation. Conclusion] This study has preliminarily clari- fied the rooting mechanism of S. microphylla cuttings. |
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| Keywords: | Syringa microphylla Auxins Cottage rooting Enzyme activity |
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