| 新疆红花品种亲缘关系的SRAP分析（英文） |
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| 引用本文： | 刘本文,唐婷婷,李彦锦,江磊,陈雁,覃瑞,刘虹,李刚.新疆红花品种亲缘关系的SRAP分析（英文）[J].农业科学与技术,2013(9):1228-1231,1270. |
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| 作者姓名： | 刘本文 唐婷婷 李彦锦 江磊 陈雁 覃瑞 刘虹 李刚 |
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| 作者单位： | 中南民族大学南方少数民族地区生物资源保护与综合利用工程中心,湖北武汉430074 |
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| 基金项目： | Supported by Natural Science Foundation of Hubei Province（2010CDZ011）; National Project for College Students to Start Innovative Career（GCX12014）~~ |
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| 摘 要： | 目的]采用SRAP分子标记技术对5个新疆红花品种进行分析,探讨品种间的亲缘关系,为红花种质资源的保护和发掘利用提供科学依据。方法]利用SRAP分子标记方法对新疆5个红花品种和1个云南红花品种的基因组DNA进行分析。结果]筛选出12对SRAP引物组合,对6份材料共扩增出171条清晰可用条带,其中多态性条带93条,多态性比率54.4%。6份材料的遗传相似系数变化范围在0.60~0.92。结论]SRAP分子标记技术适合用于红花品种的亲缘关系研究和指导分子育种。
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| 关 键 词： | 红花 亲缘关系 SRAP 聚类分析 |
SRAP Analysis on Genetic Relationship of Carthamus tinctorius L Varieties in Xinjiang |
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| Benwen LIU;Tingting TANG;Yanjin LI;Lei JIANG;Yan CHEN;Rui QIN;Hong LIU;Gang LI.SRAP Analysis on Genetic Relationship of Carthamus tinctorius L Varieties in Xinjiang[J].Agricultural Science ＆ Technology,2013(9):1228-1231,1270. |
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| Authors: | Benwen LIU;Tingting TANG;Yanjin LI;Lei JIANG;Yan CHEN;Rui QIN;Hong LIU;Gang LI |
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| Institution: | Benwen LIU;Tingting TANG;Yanjin LI;Lei JIANG;Yan CHEN;Rui QIN;Hong LIU;Gang LI;Engineering Research Centre for the Protection and Utilization of Bioresource in Ethnic Area of Southern China,South-Central University for Nationalities; |
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| Abstract: | Objective] This study aimed to analyze the genetic relationship of the Carthamus tinctorius L（safflower） varieties in Xinjiang, and thus to provide a scientific basis for its protection and development. Method] The genomic DNA of five safflower varieties from Xinjiang and one from Yunnan were analyzed and compared by SRAP molecular marker. Result] Twelve pairs of SRAP primers were selected to amplify the genomic DNA of the six materials. Among the 171 clear DNA bands finally obtained, 93 were polymorphic, accounting for 54.4% of total. Genetic similarity coefficient of the six safflower varieties ranged from 0.60 to 0.92. Conclusion]SRAP molecular marker is suitable for safflower varieties research and to guide the molecular breeding. |
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| Keywords: | Carthamus tinctorius L（safflower） Genetic relationship SRAP Cluster analysis |
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