| 铁皮石斛原球茎富铬培养条件优化（英文） |
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| 引用本文： | 杨善岩,李海龙,狄志鸿.铁皮石斛原球茎富铬培养条件优化（英文）[J].农业科学与技术,2013(12):1702-1706. |
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| 作者姓名： | 杨善岩 李海龙 狄志鸿 |
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| 作者单位： | [1]浙江亚林生物科技股份有限公司,浙江杭州311400 [2]中国林业科学研究院松花粉研究发中心,浙江杭州311400 |
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| 摘 要： | 目的]为优化铁皮石斛原球茎富铬培养工艺。方法]通过Plackett-Burman试验对时间、温度、培养基铬浓度、NAA浓度、KT浓度和光照强度等因素进行考察,结果显示时间、培养基铬浓度和光照强度3个因素具有显著效应;接着,通过显著因素的最陡爬坡试验,逼近各因素最佳值区域;最后,进行响应面优化分析。结果]最佳富铬条件为铬浓度0.37 mg/L、培养时间59 d、光照强度1 822.22 lx。原球茎铬浓度的预测值为5.08 mg/kg。结论]响应面法能对铁皮石斛原球茎富铬培养条件进行优化分析,获得最佳工艺参数,为进一步的研究奠定基础。
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| 关 键 词： | 铁皮石斛 原球茎 铬 |
Optimization of Culture Conditions for Chromium-enriched Protocorm of Dendrobium candidum |
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| Shanyan YANG,Hailong LI,Zhihong DI.Optimization of Culture Conditions for Chromium-enriched Protocorm of Dendrobium candidum[J].Agricultural Science ＆ Technology,2013(12):1702-1706. |
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| Authors: | Shanyan YANG Hailong LI Zhihong DI |
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| Institution: | 1,2 l.Zhejiang Yalin Biological Technology Co., Ltd, Hangzhou 311400, China; 2. Pine Pollen Research ＆ Development Center, Chinese Academy of Forestry, Hangzhou 311400, China |
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| Abstract: | Objective] To optimize the techniques for culturing Cr-enriched Dendrobi-um candidum protocorm. Method] By adopting Plackett-Burman test, the effects of time, temperature, Cr-content in medium, NAA content, KT content and light intensi-ty on Cr-enriched protocorm were researched. Three factors of time, Cr-content in medium and light intensity had statistical y significant effects; then steepest ascent procedures were applied to define optimal response region of these three factors; fi-nal y the optimal factors were determined by RSM analysis. Result] The results showed that the optimal conditions for Cr-enrichment were chromium concentration at 0.37 mg/L, culture time of 59 d and il umination intensity of 1 822.22 lx. The predicted value of Cr-content in protocorm was 5.08 mg/kg. Conclusion] RSM can optimize the techniques for culturing Cr-enriched protocorm of Dendrobium can-didum, provide the optimum process parameters and lay the foundation for further research. |
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| Keywords: | Dendrobium candidum Protocorm Chromium |
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