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Diallyl-disulphide to reduce the numbers of sclerotia of Sclerotium cepivorum in soil
Authors:Andrew R Entwistle  Peter R Merriman  Herbie L Munasinghe  Patricia Mitchell
Institution:1. Plant Pathology Section, National Vegetable Research Station, Wellesbourne, Warwick CV35 9EF, U.K.;2. Plant Research Institute (Department of Agriculture, Victoria), Swan Street, Burnley, Victoria 3121, Australia;3. Hatfield Polytechnic, Hatfield, Herts., U.K.
Abstract:A single injection of 0.2 ml diallyl disulphide (DADS) at 0.156% (v/v) into soil containing naturally-produced sclerotia of Sclerotium cepivorum and maintained in the laboratory at 15°C stimulated sclerotial germination and reduced sclerotial numbers by 67%; ungerminated sclerotia remained viable. Higher concentrations of DADS had no additional effect except that at 20% (v/v), germination was slightly inhibited. A similar reduction in sclerotial numbers was obtained when the mixture of soil and sclerotia was exposed to DADS vapour. Four, monthly applications of DADS at 0.2 ml 0.15% (v/v) per application did not give a further reduction.The effect of DADS was temperature dependant, with a reduction in sclerotial numbers of 65 and 9% at 15 and 5°C respectively.
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