| 纤维素酶解辅助水提黄芪多糖的工艺条件 |
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| 引用本文： | 张嘉怡,谢蓝华,杜冰,杨涛,李薇,杨公明.纤维素酶解辅助水提黄芪多糖的工艺条件[J].农产品加工．学刊,2013(2):11-14. |
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| 作者姓名： | 张嘉怡 谢蓝华 杜冰 杨涛 李薇 杨公明 |
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| 作者单位： | 华南农业大学食品学院;乌兰察布市广一中药材种植有限公司 |
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| 基金项目： | 广东省科技计划农业攻关项目(2012B020311006) |
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| 摘 要： | 以蒙古黄芪为试验材料,研究纤维素酶解辅助水提黄芪多糖的工艺条件。考察水提温度、水提时间、水提次数、料液比4个单因素对黄芪多糖提取率的影响,在单因素试验的基础上,选择4因素3水平进行正交试验优化工艺参数。结果表明,蒙古黄芪在纤维素酶解预处理后,水提温度对黄芪多糖提取率影响显著,其次是料液比和水提次数,水提时间影响较小。在料液比1:12,水提温度100℃,水提时间1.5 h,水提3次,黄芪多糖提取率达4.76%,与传统水提法(3.09%)相比,黄芪多糖提取率提高了54.05%。
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| 关 键 词： | 黄芪多糖 纤维素酶解 水提 |
Cellulase Enzymolysis Assisted Water Extraction of Astragalus Polysaccharide |
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| ZHANG Jia-yi,XIE Lan-hua,DU Bing,YANG Tao,LI Wei,YANG Gong-ming.Cellulase Enzymolysis Assisted Water Extraction of Astragalus Polysaccharide[J].Nongchanpin Jlagong.Xuekan,2013(2):11-14. |
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| Authors: | ZHANG Jia-yi XIE Lan-hua DU Bing YANG Tao LI Wei YANG Gong-ming |
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| Institution: | 1 (1.College of Food,South China Agricultural University,Guangzhou,Guangdong 510642,China; 2.Guangyi Chinese Herbal Medicine Planting Limited Company,Wulanchabu,Inner Mongolia 012000,China) |
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| Abstract: | Cellulase enzymolysis assisted water extraction of polysaccharide from Astragalus membranaceus(Fisch.) Bge.vox. mongholicus(Bge.) is optimized in the paper.To observe and study this,the effects of factors including solid-to-liquid ratio, extraction temperature,extraction time,and extraction numbers on the extraction rates of Astragalus polysaccharide are explored by single-factor and orthogonal test.The results show that extraction temperature is the most critical factor affecting the extraction rate of Astragalus polysaccharide,followed by solid-to-liquid ratio and extraction numbers,extraction time exhibited less effect on extraction rate of Astragalus polysaccharide.The optimal extraction conditions are as follows: solid-to-liquid ratio of 1:12,extraction temperature of 100℃,extraction time of 1.5 h,extraction numbers of three after cellulase enzymolysis pretreatment,Astragalus polysaccharide extraction is up to 4.76%and improving 54.05%compared with the traditional water extraction of 3.09%. |
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| Keywords: | Astragalus polysaccharide cellulase enzymolysis water extraction |
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