| 利用SSR分子标记建立杂交水稻指纹图谱 |
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| 引用本文： | 李莉,宋书锋,刘全胜,付岳峰,符习勤,戴恒.利用SSR分子标记建立杂交水稻指纹图谱[J].长江大学学报,2008,5(4). |
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| 作者姓名： | 李莉 宋书锋 刘全胜 付岳峰 符习勤 戴恒 |
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| 作者单位： | [1]中南大学隆平学院,湖南长沙410083 [2]国家杂交水稻工程技术研究中心,湖南长沙410125 [3]中国科学院亚热带农业生态研究所,湖南长沙410125 [4]湖南省岳阳市种子繁育推广中心,湖南岳阳414000 |
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| 基金项目： | 国家重点基础研究发展计划资助项目(2006CB101700) |
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| 摘 要： | 利用微卫星标记对大穗稻1126进行了DNA指纹图谱构建和品种鉴定的研究，77对引物中具有多态性的引物共36对，占所用引物的46．75％。利用这些SSR引物建立了1126的DNA指纹数据库，可以有效解决杂交水稻亲本1126的真伪性及与其他品种的区分问题。三组引物RM21、RM505、RM212共同扩增可以很好地将1126与其亲本Lemont与蜀恢527区分开来，可作为大穗稻1126的特征引物加以鉴定。由此可见利用微卫星分子标记技术进行水稻种子质量鉴定应用前景广阔。
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| 关 键 词： | 杂交水稻 SSR标记 指纹图谱 品种鉴定 |
Establishment of Fingerprint Map of Hybrid Rice with SSR Markers |
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| LI Li School of Longping Science , Technology,Central South University,Changsha,Hunan ,China,National Hybird Rice R & D Center,Hunan ,ChinaSONG Shu-feng LIU Quan-sheng,FU Yue-feng Agrotechnical Popularization Center of Yueyang of Hunan,Yueyang,Hunan ,ChinaFU Xi-qing,DAI Heng.Establishment of Fingerprint Map of Hybrid Rice with SSR Markers[J].Journal of Yangtze University,2008,5(4). |
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| Authors: | LI Li School of Longping Science Technology Central South University Changsha Hunan China National Hybird Rice R & D Center Hunan ChinaSONG Shu-feng LIU Quan-sheng FU Yue-feng Agrotechnical Popularization Center of Yueyang of Hunan Yueyang Hunan ChinaFU Xi-qing DAI Heng |
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| Institution: | LI Li School of Longping Science , Technology,Central South University,Changsha,Hunan 410083,China,National Hybird Rice R & D Center,Hunan 410125,ChinaSONG Shu-feng (Subtropic Agroecology Institute,Chinese Acadamy of Sciences,China)LIU Quan-sheng,FU Yue-feng Agrotechnical Popularization Center of Yueyang of Hunan,Yueyang,Hunan 414000,ChinaFU Xi-qing,DAI Heng (National Hybird Rice R & D Center,China) |
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| Abstract: | DNA fingerprint of hybrid rice 1126 was establelished by SSR analysis.The result showed that among 77 pairs of SSR primer,36 pairs of them showed polymorphism.The seeds of hybrid rice 1126,its parents Lemont and Shuhui 527 can be identified by the particular SSR primers RM21,RM505,RM212.It also showed that SSR technique has broad applicability in variety protection,authenticity and analysis of seed purity. |
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| Keywords: | hybrid rice SSR DNA fingerprint variety identification |
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