| 大豆引进种质抗胞囊线虫病、抗花叶病毒病和耐盐基因型鉴定及优异等位基因聚合种质筛选 |
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| 引用本文： | 叶俊华,杨启台,刘章雄,郭勇,李英慧,关荣霞,邱丽娟.大豆引进种质抗胞囊线虫病、抗花叶病毒病和耐盐基因型鉴定及优异等位基因聚合种质筛选[J].作物学报,2018,44(9):1263-1273. |
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| 作者姓名： | 叶俊华 杨启台 刘章雄 郭勇 李英慧 关荣霞 邱丽娟 |
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| 作者单位： | 东北农业大学农学院;国家农作物基因资源与遗传改良重大科学工程/农业部种质资源利用重点实验室/中国农业科学院作物科学研究所;蚌埠医学院生物科学系 |
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| 基金项目： | This study was supported by the National Key R&D Program(2016 YFD0100304);This study was supported by the National Key R&D Program(2016YFD0100304);the Agricultural Science and Technology Innovation Program of CAAS |
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| 摘 要： | 我国从美国、俄罗斯、日本等26个国家或地区共引进大豆种质3218份,仅对部分种质进行了大豆胞囊线虫病(Soybean cyst nematode,SCN)、大豆花叶病毒病(Soybean mosaic virus,SMV)和盐敏感性的抗性鉴定,但基因型的系统分析尚未见报道。本研究针对大豆抗胞囊线虫病3个基因(rhg1、Rhg4、SCN3-11)和耐盐基因(Gm SALT3)开发KASP标记5个,结合与大豆花叶病毒抗性相关的1个SCAR标记(SCN11),对1489份大豆引进种质进行基因型鉴定。结果表明,具有优异等位基因的种质共1084份;携带3个位点优异等位基因的种质19份,包括抗胞囊线虫病3个位点(rhg1、Rhg4、SCN3-11)叠加(Peking型)种质3份,聚合抗胞囊线虫病基因和抗花叶病毒病标记7份,聚合抗胞囊线虫病和耐盐基因2份,聚合抗胞囊线虫病、抗花叶病毒病和耐盐基因7份;携带4个位点优异等位基因的种质9份,包括聚合抗胞囊线虫病基因和抗花叶病毒病标记6份,聚合抗胞囊线虫病和耐盐基因2份,聚合抗胞囊线虫病、抗花叶病毒病和耐盐7份;携带5个位点优异等位基因8份,聚合了抗胞囊线虫病、抗花叶病毒病和耐盐优异等位变异。在这些携带优异等位变异的种质中,44份已由前人证明具有相应的抗性。携带3个或3个以上优异等位基因的36份种质中,有52.78%种质的一种或两种特性已被报道。在不携带抗性优异等位变异的种质中,93份已证明有耐盐性或对SMV3号株系抗性,这些种质可能存在新的抗性(等位)基因。本研究利用高通量分子标记筛选出的携带抗病、抗逆优异等位基因的种质为我国大豆资源表型鉴定、抗源的快速筛选及利用提供理论依据和新思路。
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| 收稿时间： | 2018-03-04 |
Genotyping of SCN,SMV Resistance,Salinity Tolerance and Screening of Pyramiding Favorable Alleles in Introduced Soybean Accessions |
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| Jun-Hua YE,Qi-Tai YANG,Zhang-Xiong LIU,Yong GUO,Ying-Hui LI,Rong-Xia GUAN,Li-Juan QIU.Genotyping of SCN,SMV Resistance,Salinity Tolerance and Screening of Pyramiding Favorable Alleles in Introduced Soybean Accessions[J].Acta Agronomica Sinica,2018,44(9):1263-1273. |
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| Authors: | Jun-Hua YE Qi-Tai YANG Zhang-Xiong LIU Yong GUO Ying-Hui LI Rong-Xia GUAN Li-Juan QIU |
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| Institution: | 1.College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China;2.National Key Facility for Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;3.Department of Biotechnology, Bengbu Medical College, Bengbu 233000, Anhui, China |
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| Abstract: | China has introduced 3218 soybean accessions from 26 countries such as the United States, Russia and Japan, and some of them have been carried out soybean cyst nematode (SCN), soybean mosaic virus (SMV) and salinity tolerance resistance evaluation. However, the systematic genotyping of these accessions has not been reported yet. In this study, five robust functional markers have been developed for KASP assays, three SCN loci (rhg1, Rhg4, SCN3-11) and salinity tolerance gene (GmSALT3) included. A total of 1489 introduced soybean accessions were genotyped by these markers with high-throughput assay as well as a SCAR marker (SCN11) which is related to soybean mosaic virus resistance. The results showed that there were 1084 accessions detected with favorable alleles; where accessions detected with resistant alleles at three loci were as much as 19, including three pyramiding SCN genes (rhg1, Rhg4, SCN3-11) which were Peking type and seven pyramiding SCN and SMV , two pyramiding SCN and salinity favorable alleles, as well as seven pyramiding SCN, SMV and salinity favorable alleles; and accessions detected with four favorable alleles were as much as nine accessions, including six pyramiding SCN and SMV resistance alleles, one accession detected with SCN and salinity tolerance and two detected with SCN, SMV and salinity favorable alleles, eight detected with all the favorable alleles in this study. Among the elite accessions mentioned above, it has been proved that 44 accessions resistant to SCN, SMV-3 or tolerant to salinity. Among the 36 accessions with three or more favorable alleles, 52.78% had been reported of one or two characteristics. Among the accessions without resistance or tolerance alleles, it has been reported that 93 accessions were tolerant to salinity or resistant to SMV-3, where new resistance or tolerance genes could be found. Screening out the accessions with high-throughput SNP detection assays for resistance and tolerance alleles in soybean provides information for their further phenotyping, screening and breeding. |
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| Keywords: | soybean germplasm molecular markers KASP genotyping |
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