| 不同种植方式大豆根际土壤细菌多样性分析 |
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| 引用本文： | 王芳,陈井生,刘大伟.不同种植方式大豆根际土壤细菌多样性分析[J].作物学报,2018,44(10):1539-1547. |
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| 作者姓名： | 王芳 陈井生 刘大伟 |
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| 作者单位： | 齐齐哈尔大学生命科学与农林学院/抗性基因工程与寒地生物多样性保护黑龙江省重点实验室;黑龙江省农科院大庆分院;东北农业大学农学院 |
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| 基金项目： | This study was supported by the Heilongjiang Provincial Higher-education Basic Scientific Research Project(2012K-M21) |
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| 摘 要： | 利用Illumina MiSeq第二代高通量测序平台,对黑龙江省不同地区轮作及连作大豆根际土壤细菌16S rDNA基因组测序,初步分析在不同种植方式下受大豆胞囊线虫侵染的大豆根际土壤细菌群落结构的变化。从6个土壤样本中共获得25 419个OTUs,鉴定到细菌的47个门, 147个纲,709个属。变形菌门、放线菌门、酸杆菌门、芽单胞菌门、浮霉菌门是供试土壤细菌的优势菌门,占所有细菌群落总数90%以上。连作4年总OTUs及丰富度最高,连作20年最低。不同轮作方式土壤细菌丰富度差异不显著(P 0.05),短期连作与长期连作细菌丰富度及多样性差异显著(P 0.05)。不同轮作方式下放线菌门相对丰度低于连作方式,芽单胞菌门和拟杆菌门相对丰度高于同地区连作方式。土壤功能细菌根瘤菌(Bradyrhizobium)、链霉菌属(Streptomyces)、芽孢杆菌属(Bacillus)、溶杆菌属(Lysobacter)、土微菌属(Pedomicrobium)的相对丰度在不同年限连作下高于轮作。长期连作土壤优势细菌丰度与轮作土壤相似性更高。
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| 收稿时间： | 2018-02-05 |
Bacterial Diversity of Soybean Rhizosphere Soil under Different Cropping Patterns |
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| Fang WANG,Jing-Sheng CHEN,Da-Wei LIU.Bacterial Diversity of Soybean Rhizosphere Soil under Different Cropping Patterns[J].Acta Agronomica Sinica,2018,44(10):1539-1547. |
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| Authors: | Fang WANG Jing-Sheng CHEN Da-Wei LIU |
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| Institution: | 1.College of Life Sciences and Agroforestry, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihar 161006, Heilongjiang, China;2.Daqing Branch of Heilongjiang Academy of Agricultural Sciences, Daqing 163316, Heilongjiang, China;3.Agronomy College, Northeast Agricultural University, Harbin 150030, Heilongjiang, China |
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| Abstract: | To study bacterial community structure of soybean rhizosphere soil in rotation and continuous cropping, 16S rDNA gene were sequenced of soil samples infected soybean cyst nematode from Heilongjiang Province two regions by the second generation of high-throughput sequencing Illumina MiSeq platform. A total of 25 419 OTUs were obtained from six soil samples and classified into 47 phylum, 147 class, and 709 genera. Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, Planctomycetes were dominant bacteria, accounting for more than 90% of all soil bacterial communities. The total OTUs and richness were the highest in four years continuous cropping and the lowest in twenty years continuous cropping. The difference of bacteria abundance in different rotational cropping years was not obvious (P > 0.05), but abundance and adversity were significant in continuous cropping (P < 0.05). The relative abundance of Actinobacteria in different rotations was lower than that in continuous cropping, and the relative abundance of Gemmatimonadetes and Bacteroidetes was higher than that in the same area. The relative abundance of soil functional bacteria Bradyrhizobium, Streptomyces, Bacillus, Lysobacter, and Pedomicrobium were higher in different continuous cropping years than those in rotations. Predominant bacterial abundance in long term continuous cropping was more similar with that in rotational cropping. |
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| Keywords: | soybean rotation continuous cropping bacterial diversity Illumina MiSeq sequencing |
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