| 芥菜型油菜毛状根诱导体系构建及TTG1基因功能初步研究 |
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| 引用本文： | 李隆,程成,伍小方,张大为,刘丽莉,周静,周美亮,张凯旋,严明理.芥菜型油菜毛状根诱导体系构建及TTG1基因功能初步研究[J].作物学报,2018,44(10):1468-1476. |
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| 作者姓名： | 李隆 程成 伍小方 张大为 刘丽莉 周静 周美亮 张凯旋 严明理 |
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| 作者单位： | 湖南科技大学生命科学学院;中国农业科学院作物科学研究所;湖南科技大学重金属污染土壤生态修复与安全利用湖南省普通高等学校重点实验室 |
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| 基金项目： | This study was supported by the National Key Research and Development Program(2016YFD0100202);the Natural Science Foundation of Hunan Province(2016JJ1010);the National Natural Science Foundation of China(31572457);the Foundation of Hunan Education Department(17K035) |
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| 摘 要： | 黄籽油菜具有种皮薄、出油率高等优点,研究油菜黄籽的形成具有重要的意义。前期研究表明,TTG1(TRANSPARENT TESTA GLABRA 1)基因参与了油菜种皮颜色的形成。本研究利用发根农杆菌A4菌株诱导了芥菜型油菜四川黄籽的毛状根,并研究了菌液浓度和外植体部位对诱导率的影响。结果表明,菌液浓度OD值为0.8时,诱导效率最高,平均为71.5%;外植体中下胚轴的发根率最高,平均为87.3%。在四川黄籽毛状根中过表达TTG1基因,发现原花色素合成途径中的DFR(dihydroflavonol4-reductase)、ANS(anthocyanidinsynthase)和BAN(anthocyanidin reductase)基因的表达受到抑制。本研究优化了油菜毛状根的诱导体系,为利用毛状根体系进行基因功能验证提供了新的思路和方法。
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| 收稿时间： | 2018-02-14 |
Construction of Hairy Root Induction System and Functional Analysis of TTG1 Gene in Brassica juncea |
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| Long LI,Cheng CHENG,Xiao-Fang WU,Da-Wei ZHANG,Li-Li LIU,Jing ZHOU,Mei-Liang ZHOU,Kai-Xuan ZHANG,Ming-Li YAN.Construction of Hairy Root Induction System and Functional Analysis of TTG1 Gene in Brassica juncea[J].Acta Agronomica Sinica,2018,44(10):1468-1476. |
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| Authors: | Long LI Cheng CHENG Xiao-Fang WU Da-Wei ZHANG Li-Li LIU Jing ZHOU Mei-Liang ZHOU Kai-Xuan ZHANG Ming-Li YAN |
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| Institution: | 1.College of Life Science, Hunan University of Science and Technology, Xiangtan 411201, Hunan, China;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;3.Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, Hunan University of Science and Technology, Xiangtan 411201, Hunan, China |
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| Abstract: | Yellow seed rape has the advantages of thin seed coat and high oil yield. It is of great significance to study the formation of yellow seed in rapeseed. Previous studies showed that TTG1 (TRANSPARENT TESTA GLABRA 1) gene was involved in the formation of seed coat color. In this study, Agrobacterium rhizogenes A4 strain was used to induce hairy roots of Sichuan yellow, and investigate the effects of bacterial concentration and different sources of explants on hairy root induction. The highest average rooting rate was 71.5% when the OD value of A. rhizogenes A4 reached to 0.8. The average rooting rate of hypocotyls was significantly higher than that of cotyledons and up to 87.3% after the infection. Overexpression of TTG1 gene in Sichuan yellow hairy roots inhibited the expression of DFR (Dihydroflavonol4-reductase), ANS (Anthocyanidin synthase), and BAN (Anthocyanidin reductase) genes, encoding key enzymes of the proanthocyanidins biosynthesis. In this study, we optimized the induction system of hairy roots in B. juncea, and analyzed the function of TTG1 in the regulation of proanthocyanidins synthesis pathway, which provide a new idea and method for the functional analysis of genes in the hairy root system. |
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| Keywords: | Brassica juncea hairy root inducing condition TTG1 gene proanthocyanidins |
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