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湖北省规模化猪场猪繁殖与呼吸综合征血清学检测
引用本文:杨克礼,田永祥,段正赢,周丹娜,郭 锐,刘泽文,袁芳艳,孟 丽.湖北省规模化猪场猪繁殖与呼吸综合征血清学检测[J].中国农学通报,2015,31(2):31-34.
作者姓名:杨克礼  田永祥  段正赢  周丹娜  郭 锐  刘泽文  袁芳艳  孟 丽
作者单位:湖北省农业科学院畜牧兽医研究所,湖北省农业科学院畜牧兽医研究所,湖北省农业科学院畜牧兽医研究所,湖北省农业科学院畜牧兽医研究所,湖北省农业科学院畜牧兽医研究所,湖北省农业科学院畜牧兽医研究所,湖北省农业科学院畜牧兽医研究所,湖北省农业科学院畜牧兽医研究所
基金项目:基金项目:湖北省农业科技创新中心项目“生猪主要疫病防控技术研究与示范”(2011-620-001-003);湖北省重点自然科学基金“猪繁殖与呼吸综合征病毒野毒与疫苗株(JXA1-R)鉴别荧光定量RT-PCR方法研究”(2012FFA067);湖北省重点新产品新工艺研究开发项目“规模化猪场猪繁殖与呼吸综合征综合防控技术的研究与示范”(2012BBA15004);动物胚胎及分子育种湖北省重点实验室开放课题“猪繁殖与呼吸综合征快速鉴别诊断方法研究”(2013ZD156)。
摘    要:为了解湖北省内规模化养猪场猪繁殖与呼吸综合征的感染及疫苗免疫状况,在省内17个地市的50个规模化猪场采集5160份血清样品进行了PRRSV抗体检测。结果显示:未免疫猪场PRRSV抗体平均阳性率为33.54%(21.10%~46.92%),其中公猪的阳性率为0,母猪的阳性率为7.69%,而育肥猪、保育猪及哺乳仔猪的阳性率分别为34.24%、28.18%和38.53%;免疫猪场PRRSV抗体平均阳性率为75.52%(68.46%~100%),其中公猪的阳性率为100%,母猪的阳性率为96.78%,育肥猪、保育猪及哺乳仔猪的阳性率分别为70.07%、74.28%和68.46%。说明PRRS在湖北省普遍流行,且感染程度高,流行范围广,应引起足够的重视。

关 键 词:PRRSV  血清学  检测  ELISA
收稿时间:2014/6/23 0:00:00
修稿时间:2014/12/11 0:00:00

Serological Detection of Porcine Reproductive and Respiratory Syndrome in Large-scale Swine Farms in Hubei Province
Yang Keli,Tian Yongxiang,Duan Zhengying,Zhou Dann,Guo Rui,Liu Zewen,Yuan Fangyan and Meng Li.Serological Detection of Porcine Reproductive and Respiratory Syndrome in Large-scale Swine Farms in Hubei Province[J].Chinese Agricultural Science Bulletin,2015,31(2):31-34.
Authors:Yang Keli  Tian Yongxiang  Duan Zhengying  Zhou Dann  Guo Rui  Liu Zewen  Yuan Fangyan and Meng Li
Institution:Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Hubei Key Laboratory of Animal Embryo and Molecular Breeding
Abstract:5160 porcine sera, which were collected from 50 farms in Hubei province, were detected antibody against PRRSV by ELISA. The results showed that the average PRRSV positive rate of non-immunized farms was 33.54% (21.10%-46.92%), the positive rate of farms was 100%, and the average PRRSV positive rate of immunized farms was 75.52% (68.46%-100%). The positive rate of boar, sow, fattening pigs, weanling pigs and suckling piglets was 0, 7.69%, 34.24%, 28.18%, and 38.53% in non-immunized farms; and 100%, 96.78%, 70.07%, 74.28% and 68.46% in immunized farms, respectively. The results indicated that subclinical infection was widespread and seriously harmful in Hubei province.
Keywords:PRRSV  serology  detection  ELISA
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