| 散穗高粱与白壳苏丹草杂交F1及其染色体加倍植株ISSR分析 |
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| 引用本文： | 周亚星,周伟.散穗高粱与白壳苏丹草杂交F1及其染色体加倍植株ISSR分析[J].中国农学通报,2015,31(3):249-252. |
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| 作者姓名： | 周亚星 周伟 |
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| 作者单位： | 内蒙古民族大学农学院,内蒙古民族大学 |
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| 基金项目： | 内蒙古自然科学基金项目“散穗高粱×白壳苏丹草杂种F1染色体加倍及选育研究”(2013MS0405)；内蒙古民族大学博士启动基金项目“散穗高粱与苏丹草杂交后代优良株系选育”(BS276)。 |
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| 摘 要： | 为了明确散穗高粱与白壳苏丹草杂交F1及其染色体加倍植株遗传差异性及其遗传距离，为下一步新品种育成利用奠定基础和提供科学依据。试验利用ISSR标记技术对散穗高粱与白壳苏丹草杂交F1及其染色体加倍植株进行多态性位点比较分析。结果表明：6条适宜引物共扩增得到重复性好的清晰条带164条，其中144条多态性条带，多态性比率达到86.9%；材料间的遗传距离变幅为0.2690~0.5923，散穗高粱与散穗高粱×白壳苏丹草F1加倍植株之间的遗传距离最远；供试材料以遗传距离0.26为基准可分为散穗高粱和白壳苏丹草；散穗高粱×白壳苏丹草杂种F1；散穗高粱×白壳苏丹草杂种F1加倍植株这三类。
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| 关 键 词： | 衡阳 衡阳 |
| 收稿时间： | 8/6/2014 12:00:00 AM |
| 修稿时间： | 8/6/2014 12:00:00 AM |
ISSR Analysis on Sorghum-White Hull Sudangrass of Cross F1 and Its Chromosome Doubling Plants |
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| Zhou Yaxing;Zhou Wei.ISSR Analysis on Sorghum-White Hull Sudangrass of Cross F1 and Its Chromosome Doubling Plants[J].Chinese Agricultural Science Bulletin,2015,31(3):249-252. |
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| Authors: | Zhou Yaxing;Zhou Wei |
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| Institution: | Zhou Yaxing;Zhou Wei;College of Agriculture, Inner Mongolia University for Nationalities;Crop Research Institute, Inner Mongolia University for the Nationalities; |
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| Abstract: | The paper aims to clarify genetic differences and genetic distance of sorghum-white hull sudangrass of cross F1 and chromosome doubling plant of sorghum-white hull sudangrass hybrids F1 and lay the foundation and provide a scientific basis for further breeding of new varieties. ISSR markers for sorghum- white hull sudangrass of cross F1 and chromosome doubling plant of sorghum-white hull sudangrass of cross F1 were used to carry out the comparative analysis of polymorphic loci. The results showed that, ISSR markers were amplified with 6 suitable primers and 164 bands were obtained, among which the bands of polymorphic were 144, accounted for 86.9%. The genetic distance was ranged from 0.2690 to 0.5923, and the genetic distance between sorghum and doubling plant of sorghum-white hull sudangrass of cross F1 was further. Using the genetic distance value of 0.26 as threshold, the test materials could be divided into 3 kinds: sorghum and white hull sudangrass, sorghum- white hull sudangrass of cross F1 and doubling plant of sorghum- white hull sudangrass of cross F1. |
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| Keywords: | sorghum white hull sudangrass chromosome doubling plant ISSR |
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