| 环境诱变剂诱变的家蚕核型多角体病毒继代实验及诱变病毒基因组的酶切分析 |
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| 引用本文： | 曹阳,黄自然,黄亚东,徐兴耀,刘良式.环境诱变剂诱变的家蚕核型多角体病毒继代实验及诱变病毒基因组的酶切分析[J].蚕业科学,1999,25(1). |
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| 作者姓名： | 曹阳 黄自然 黄亚东 徐兴耀 刘良式 |
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| 作者单位： | 1. 华南农业大学蚕桑系,广州,510642
2. 中山大学生命科学学院 |
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| 摘 要： | 前文报道环境诱变剂丝裂霉素Ｃ（ＭＭＣ）、９ 氨基吖啶（９ ＡＡ）和甲基磺酸乙酯（ＥＭＳ）对家蚕核型多角体病毒（ＢｍＮＰＶ）多角体形态有明显的诱变作用。本文进一步报道形态变异的ＢｍＮＰＶ多角体在继代实验中的仍保持相对稳定的比率［（２１３±４２８）％～（３００±４８５）％］，说明诱变的异常多角体能较稳定地遗传到次代，ＭＭＣ及９ ＡＡ诱变的ＢｍＮＰＶ ＤＮＡ，经ＥｃｏＲⅠ、ＢａｍＨⅠ和ＢｇｌⅡ酶切的电泳图谱与野生型对照株相比较，出现某些ＤＮＡ泳带的增加或丢失。显示诱变的ＢｍＮＰＶ基因组可能发生了多处高频率的碱基热点序列的改变
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| 关 键 词： | 环境诱变剂 家蚕核型多角体病毒 诱变 |
The Successive Test of the Environmental-mutagen-mutated Bombyx mori Nuclear Polyhedrosis Virus and the Restriction Analysis of Its Genome |
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| Abstract: | In our early studies,Mitomycine (MMC),Aminoacridine (9 AA) and Ethyl methanesulfnonate (EMS) were used as environmental mutagens to Bombyx mori nuclear polyhedrosis virus (BmNPV).They distinctly induced the morphological change of polyhedra. The present studies showed that the variant polyhedra had relative stability in the next generation with high rate about (21.3±4.28)%～(30.0±4.85)%].The BmNPV DNA of MMC and 9 AA mutants were digested by EcoR Ⅰ,BamH Ⅰ and Bgl Ⅱ,and some additional DNA bands appeared and some disappeared on the electropherogram as compared with the control of the BmNPV DNA.This shows that mutation of BmNPV genome had occurred at some hotspots. |
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| Keywords: | Enviromental Mutagens BmNPV Mutagenesis |
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