| 大孔吸附树脂提取多杀菌素的方法 |
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| 引用本文： | 胡西洲,贺玉平,戴经元,林开春.大孔吸附树脂提取多杀菌素的方法[J].华中农业大学学报,2006,25(4):397-399. |
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| 作者姓名： | 胡西洲 贺玉平 戴经元 林开春 |
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| 作者单位： | 1. 武汉天惠生物工程有限公司,武汉,430070;华中农业大学植物科技学院,武汉,430070
2. 武汉天惠生物工程有限公司,武汉,430070 |
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| 摘 要： | 采用大孔树脂吸附法提取多杀菌素。通过树脂选型,筛选出XAD-4作为多杀菌素提取的吸附剂;吸附最适pH值为11.0,流速为1/6(1/min),加入2%氯化钠;采用丙酮梯度解吸,总收率达64.7%。
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| 关 键 词： | 多杀菌素 大孔吸附树脂 分离纯化 |
| 文章编号： | 1000-2421（2006）04-0397-03 |
| 收稿时间： | 05 24 2006 12:00AM |
| 修稿时间： | 2006-05-242006-06-26 |
Isolation and Purification of Spinosyns by Macroreticular Resin Adsorbent Method |
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| HU Xi-zhou,HE Yu-ping,DAI Jing-yuan,LIN Kai-chun.Isolation and Purification of Spinosyns by Macroreticular Resin Adsorbent Method[J].Journal of Huazhong Agricultural University,2006,25(4):397-399. |
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| Authors: | HU Xi-zhou HE Yu-ping DAI Jing-yuan LIN Kai-chun |
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| Abstract: | In this dissertation,process of isolating and purifying spinosad from fermentation broth by macroreticular adsorbent resin was determined.XAD-4 nonpolar resin was screened as the best adsorbent,and adsorbent conditions were optimized under the following conditions: the pH was maintained at 11,flow rate of adsorption was 1/6(1/min),2.0% NaCl was feasible,and acetone was used for eluting spinosad.As a result,the recovery rate was 64.7%. |
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| Keywords: | spinosys marcoreticular resin isolation and purification |
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