| 百合辐射突变体的ISSR鉴定技术研究 |
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| 引用本文： | 孙利娜,席梦利,徐进,吴祝华,施季森.百合辐射突变体的ISSR鉴定技术研究[J].现代农业科技,2011(1):219-221. |
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| 作者姓名： | 孙利娜 席梦利 徐进 吴祝华 施季森 |
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| 作者单位： | 孙利娜,SUN Li-na(南京林业大学林木遗传和生物技术省部共建教育部重点实验室,江苏南京,210037;广西壮族自治区林业科学研究院);席梦利,徐进,吴祝华,施季森,XI Meng-li,XU Jin,WU Zhu-hua,SHI Ji-sen(南京林业大学林木遗传和生物技术省部共建教育部重点实验室,江苏南京,210037) |
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| 基金项目： | 国家林业局"948"引进项目 |
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| 摘 要： | 用60Co～γ射线辐射麝香百合WhiteFox的鳞片获得变异后代,从分子水平上检测60Co～γ射线对WhiteFox遗传物质的影响。运用ISSR分子标记方法对12株变异株和对照进行分析研究,从20条引物中筛选出5条具有多态性的引物进行PCR扩增,共检测到29个位点,其中多态位点18个,占62.1%。结果表明:经过60Co～γ射线辐射获得的变异百合在DNA水平上存在多态性差异。
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| 关 键 词： | 百合 辐射 突变体 ISSR |
Research of ISSR Identifying Technology of Lily Radiation Mutants |
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| Authors: | SUN Li-na XI Meng-li XU Jin WU Zhu-hua SHI Ji-sen |
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| Institution: | SUN Li-na 1,2 XI Meng-li 1 XU Jin 1 WU Zhu-hua 1 SHI Ji-sen 1 (1 The Key Laboratory of Forest Genetic and Biotechnology,Ministry of Education,Nanjing Forestry University,Nanjing Jiangsu 210037,2 Guangxi Forestry Research Institute) |
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| Abstract: | Using 60Co～gamma ray radiated the scales of White Fox of musk lily and gained variation offsprings and detected the influence on genetic material of White Fox at the molecular level.Inter simple sequence repeat(ISSR)method was used to analyze the 12 variation offsprings and the contrast strains.5 primers screened out of 20 were amplified and the amplification 29 sites were obtained,of which,18 were polymorphic loci and the percentage of polymorphic bands was 62.1%.The results showed that after 60Co~gamma ray radiate the variation of lily existed polymorphism differences in DNA level. |
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| Keywords: | ISSR |
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