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| 京海黄鸡IGF-IR基因外显子3多态性及遗传效应分析 | |
| 引用本文： | 杨凤萍,金崇富,俞亚波,谢凯舟,戴国俊,王金玉.京海黄鸡IGF-IR基因外显子3多态性及遗传效应分析[J].畜牧与兽医,2013,45(7):1-5. |
| 作者姓名： | 杨凤萍 金崇富 俞亚波 谢凯舟 戴国俊 王金玉 |
| 作者单位： | 1. 扬州大学生物科学与技术学院,江苏扬州,225009 2. 扬州大学动物科学与技术学院,江苏扬州,225009 3. 江苏京海禽业集团有限公司,江苏海门,226103 |
| 摘 要： | 结合PCR-SSCP与PCR-RFLP技术,检测分析了京海黄鸡母鸡IGF-IR基因第3外显子SNPs的特点及其对京海黄鸡母鸡生产性状的遗传效应。结果在该段序列检测到2个点突变:P1(G11 0996C)、P2(C11 1014A),2位点均表现出3种基因型,其中P1位点偏离Hardy-Weinberg平衡状态。最小二乘方差分析表明,P2位点AA型个体初生重显著高于CC、CA型个体(P<0.05)。联合基因型分析表明,GC/AA基因组合型母鸡开产较晚但开产蛋重较高;CC/CA型个体,其12周龄、300日龄体重极显著低于CC/AA型个体(P<0.01),初生重、4周龄、12周龄、16周龄和300日龄体重显著低于其他基因组合型个体(P<0.05);CC/AA型个体12周龄、300日龄体重极显著高于GC/CC、CC/CA型个体(P<0.01),16周龄体重显著高于GC/CC、GC/CA、GC/AA、CC/CA型(P<0.05)。利用基因组合型GC/AA、CC/CA、CC/AA与生产性能间的关联特性指导京海黄鸡,能够为利用分子标记加快京海黄鸡的保种和育种进程提供有益参考。 |
| 关 键 词： | 京海黄鸡 IGF-IR SNPs 生产性能 遗传效应 |
Polymorphisms of IGF-IR gene exon3 and theirs genetic effect analysis in Jinghai yellow chickens |
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| YANG Fengping , JIN Congfu , YU Yabo , XIE Kaizhou , DAI Guojun , WANG Jinyu.Polymorphisms of IGF-IR gene exon3 and theirs genetic effect analysis in Jinghai yellow chickens[J].Animal Husbandry & Veterinary Medicine,2013,45(7):1-5. | |
| Authors: | YANG Fengping JIN Congfu YU Yabo XIE Kaizhou DAI Guojun WANG Jinyu |
| Institution: | 1.College of Bioscience and Technology,Yangzhou University,Yangzhou 225009,China; 2.College of Animal Science and Techology,Yangzhou University,Yangzhou 225009,China; 3.Jiangsu Jinghai Poultry Group Co.,Ltd,Haimen 226103,China) |
| Abstract: | The SNPs of IGF-IR exon3 were studied by PCR-SSCP and PCR-RFLP in Jinghai yellow chicken and their genetic effects on production performances were also analyzed.The results showed that 2 SNPs at G11 0996C and C11 1014A were identified and three genotypes were detected in each locus,P1 locus deviated the Hardy-Weinberg equilibrium(P<0.05).The Least Square Means analysis showed that individuals with AA genotype had higher body weight than those with CC and CA at 1-day-age(P<0.05).Hens with GC/AA had late first-egg-age but high first-egg-weight.Individuals with CC/CA com-genotype had low body weight,which was significantly lower than those with CC/AA at 12-weeky-day and 300-day(P<0.01),lower than any other com-genotypes at 1-day,4-weeky-day,12-weeky-day,16-weeky-day and 300-day(P<0.05).Those with CC/AA had significantly higher body weight than those with GC/CC or CC/CA at 12-weeky-day and 300-day(P<0.01),higher than those with GC/CC,GC/CA,GC/AA,CC/CA(P<0.05),respectively.The correlation characteristics between GC/AA,CC/CA,CC/AA and production performances could be helpful to accelerate the conservation and breeding process of Jinghai yellow chicken. |
| Keywords: | Jinghai yellow chicken IGF-IR SNPs production performance genetic effect |
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