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南非羊蹄甲的离体培养和植株再生
引用本文:吕秀立,张庆费,边黎明,王锐,徐闪峰,桂仁意.南非羊蹄甲的离体培养和植株再生[J].林业科学研究,2008,21(3):407-410.
作者姓名:吕秀立  张庆费  边黎明  王锐  徐闪峰  桂仁意
作者单位:1. 上海市园林科学研究所,上海,200232;南京林业大学林木遗传和基因工程重点实验室,江苏,南京,210037
2. 上海市园林科学研究所,上海,200232
3. 南京林业大学林木遗传和基因工程重点实验室,江苏,南京,210037
4. 上海市林业总站,上海,200072
5. 浙江林学院林业与生物技术学院,浙江,临安,311300
基金项目:国家科技支撑计划,上海市科学技术委员会登山计划
摘    要:南非羊蹄甲(Bauhinia galpinii)为豆科(Leguminosae)羊蹄甲属(Bauhinia L.)植物.该属植物世界分布约600余种,遍布于热带地区,我国有40种,生长于南部和西南部1],为灌木、藤本及小乔木,是深根性树种,易栽培难移栽,抗炎热耐干旱贫瘠土壤,绝大多数种类不耐寒.

关 键 词:南非羊蹄甲  离体培养  植株再生
收稿时间:2007/10/8 0:00:00

In V itro Culture and Plant Regenera tion of B auh in ia ga lpin ii
LU Xiu-li,ZHANG Qing-fei,BIAN Li-ming,WANG Rui,XU Shan-feng and GUI Ren-yi.In V itro Culture and Plant Regenera tion of B auh in ia ga lpin ii[J].Forest Research,2008,21(3):407-410.
Authors:LU Xiu-li  ZHANG Qing-fei  BIAN Li-ming  WANG Rui  XU Shan-feng and GUI Ren-yi
Abstract:Winter lignified stems of B auhinia galpinii were used as exp lants for tissue culture, and the effects ofdifferent hormones on inductivity rate and rooting rate were investigated by supp lementing different concentrations.The results indicated that budswere induced from a 2 cm high young p lantlet cultured inMSmedium supp lementedwith 1. 0 mg·L-1 62BA and 0. 1 mg·L-1 NAA for 20 d, and the induction rate was 100%. The mean amount ofbuds was 8. 7. The medium ofMS + 0. 4mg·L-1 NAA was used for rooting and the rooting rate was 85%. Theexperiment strategy to establish tissue culture system from lignified stem was feasible, which could be used for coldacclimation and cold resistantmutant selection.
Keywords:B auhinia galpinii  in vitro culture  p lant regeneration
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