Cytochemical investigation of long-term stored maize pollen |
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| Authors: | I D Georgieva M M Kruleva |
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| Institution: | (1) Institute of Genetics, 1113 Sofia, Bulgaria |
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| Abstract: | Summary Maize pollen quality was investigated after long-term storage both in a refrigerator and in liquid nitrogen by a combination of viability tests and cytochemical methods. Determination of the activities of a number of enzymes involved in important metabolic pathways was carried out. Quinone formation was also studied, as some products of secondary metabolism affect pollen grain viability. One year of pollen storage in liquid nitrogen had little effect on the activities of oxidoreductases and hydrolases and had no significant effect on pollen grain viability evaluated by acetocarmine, neutral red and acridine organe. Only the FCR test showed slightly decreased viability. After one and two years of storage in a refrigerator, pollen grain viability, tested using acetocarmine, neutral red and acridine orange, did not change substantially. Simultaneously the FCR test showed a considerable decrease in pollen grain viability. Long-term storage in a refrigerator resulted in the loss of cytochrome oxidase activity and rise of alcohol dehydrogenase, lactate dehydrogenase, peroxidase and polyphenoloxidase activities as well as of quinone formation.Abbreviations ADH
Alcohol dehydrogenase
- DOPA
L-3,4-Dihydroxyphenylalanine
- FCR
Fluorochromatic reaction
- FDA
Fluorescein diacetate
- GDH
Glutamate dehydrogenase
- IDH
Isocitrate dehydrogenase
- LDH
Lactate dehydrogenase
- NADI
reaction with  -NAphthol and DImethyl-p-phenylenediamine hydrochloride
- 6-PGDH
6-phosphogluconate dehydrogenase |
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| Keywords: | Zea mays maize pollen storage pollen grain viability pollen enzymes pollen physiology enzyme cytochemistry |
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