| 水稻白叶枯病黄单胞菌编码顺乌头酸酶的rpfA基因的鉴定 |
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| 引用本文： | 武波,唐纪良,马庆生.水稻白叶枯病黄单胞菌编码顺乌头酸酶的rpfA基因的鉴定[J].广西农业生物科学,1996(1). |
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| 作者姓名： | 武波 唐纪良 马庆生 |
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| 作者单位： | 广西农业大学实验中心分子遗传学研究室，英国Ｓａｉｎｓｂｕｒｙ实验室 |
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| 基金项目： | 国家863高技术研究计划资助 |
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| 摘 要： | 通过生化功能分析，证实了水稻白叶枯病黄单胞菌野生型菌株Ｔ３０００产生两种顺乌头酸酶（分别命名为ＸｏｏＡｃａｎＡ和ＸｏｏＡｃａｎＢ）。通过三亲本接合的方法，将获得的含水稻白叶枯病黄单胞菌ｒｐｆ基因簇的重组质粒ｐＧＸＮ３０００导入甘蓝黑腐病黄单胞菌ｒｐｆＡ基因突变体８４７６中。经生化功能分析证实，８４７６／ｐＧＸＮ３０００中含有水稻白叶枯病黄单胞菌的顺乌头酸酶ＸｏｏＡｃａｎＡ；同时还证实了ＰＧＸＮ３０００能互补８４７６，恢复其合成胞外酶和胞外多糖的能力及致病性．这表明在ＰＧＸＮ３０００中含有一个完整的水稻白叶枯病黄单胞菌的顺乌头酸酶基因，该基因具有类似甘蓝黑腐病黄单胞菌ｒｐｆＡ基因的功能．因此将该基因命名为水稻白叶枯病黄单胞菌ｒｐｆＡ基因。
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| 关 键 词： | 水稻白叶枯病黄单胞菌 甘蓝黑腐病黄单胞菌 rPfA基因 顺乌头酸酶 |
Identification of rpfA gene of Xanthomonas oryzae pv. oryzae |
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| Wu Bo, G. T. J. Wilson, Tang Jiliang, Ma Qingshan, M. J. Daniels.Identification of rpfA gene of Xanthomonas oryzae pv. oryzae[J].Journal of Guangxi Agricultural and Biological Science,1996(1). |
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| Authors: | Wu Bo G T J Wilson Tang Jiliang Ma Qingshan M J Daniels |
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| Abstract: | it was demonstrated that Xanthomonas Oryzae pv. oryzae (Xoo), the causal agent of bacterial blight disease of rice, produces two aconitases designated XooAcanA and XooAcanB, respectively. A recombinant plasmid PGXN3000 containing a cluster genes of regulation of pathogenicity factor (rpf genes) of Xoo, was introduced into strain 8476, a mutant of rpfA gene of Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot of crucifers. It was shown that PGXN3000 contains an aconitase gene encoding XooAcanA and could express in strain 8476. This aconitase gene which was referred to as rpfA gene of Xoo was shown to restore the pathogenicity and production of extracellular enzymes and polysaccharide of strain 8476. |
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| Keywords: | Xanthomonas oryzae pv oryzae Xanthomonas campestris pv campestris rpfA gene aconitase |
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