Fungal and plant gene expression during synchronized infection of tomato leaves by Botrytis cinerea |
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| Authors: | Ernesto P Benito Arjen ten Have John W van 't Klooster Jan AL van Kan |
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| Institution: | (1) Department of Phytopathology, Wageningen Agricultural University, P.O. Box 8025, 6700 EE Wageningen, The Netherlands |
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| Abstract: | An inoculation procedure was developed to obtain efficient and synchronous infection on detached tomato leaves by Botrytis cinerea. In spray-inoculated leaves incubated at 20 °C, the infection process consisted of three phases: the formation of primary necrotic lesions (until 20 hpi), a quiescent phase (20-72 hpi), and the expansion of a proportion of the primary lesions (from 72 hpi onwards), resulting in full tissue maceration. At 4 °C, the infection progressed slowly but steadily without inducing necrotic responses in the host. The actin and  -tubulin genes of B. cinerea were cloned, characterized and used as probes on blots containing RNAs from leaves at various stages of the infection. The genes displayed a similar expression pattern throughout the infection and the hybridization signal reflected the amount of fungal biomass. The actin mRNA accumulated to higher levels than the -tubulin mRNA. Tomato PR protein mRNAs (chitinase, -1,3-glucanase and PR-1) were induced during the infection, albeit with different kinetics and to different levels. At 20 °C, -1,3-glucanase and PR-1 mRNAs were induced more rapidly than chitinase mRNAs. At 4 °C, mRNAs encoding extracellular -1,3-glucanase and intracellular, as well as extracellular chitinase were hardly induced. |
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| Keywords: | actin fungal biomass grey mould necrosis PR proteins  -tubulin" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-tubulin |
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