| 木薯淀粉分支酶活性测定方法比较研究 |
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| 引用本文： | 郭运玲,孔华,左娇,黄启星,贾瑞宗,郭静远,周霞,郭安平.木薯淀粉分支酶活性测定方法比较研究[J].中国农学通报,2016,32(33):193-198. |
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| 作者姓名： | 郭运玲 孔华 左娇 黄启星 贾瑞宗 郭静远 周霞 郭安平 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所,中国热带农业科学院热带生物技术研究所 |
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| 基金项目： | 海南省应用技术研究与开发专项项目“能源专用型高产高直链淀粉木薯品种的培育与应用”(ZDXM2014051)；国家自然科学基金项目“木 薯淀粉品质改良的转基因关键技术研究”(30960204)。 |
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| 摘 要： | 旨在找到木薯淀粉分支酶活性有效测定方法。以成熟期的木薯块根为材料,对现有的淀粉分支酶活性测定方法从酶液提取液、酶液浓度、反应体系、反应时间、到酶反应终止方式作了比较和改进。木薯淀粉分支酶活性测定的有效方法：取0.5g成熟木薯块根,加入提取液研磨成浆,离心后上清为粗酶液,粗酶液稀释后加0.5%可溶性淀粉,37℃恒温水浴中保温10 min后置沸水浴中终止反应,再加碘液显色反应10min后测定波长660nm处吸光度值,木薯淀粉分支酶SBE活性以波长660 nm的吸光度下降百分率表示,以每降低1％碘蓝值为一个酶活性单位(U)。改良后的淀粉分支酶活性测定方法能很好的区分不同木薯株系淀粉分支酶活性差异,实验重复性好,操作简单、快捷。
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| 关 键 词： | 层状云系发展阶段 层状云系发展阶段 云微物理特征 飞机探测 降水机制 |
| 收稿时间： | 2016/4/29 0:00:00 |
| 修稿时间： | 2016/10/25 0:00:00 |
Comparison of Activity Measuring Methods of Cassava Starch Branching Enzyme |
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| Abstract: | An effective method for measuring activity of starch branching enzyme was developed in this study. Tuberous roots of mature cassava were collected as materials to study various reaction parameters of the starch branching enzyme (SBE), including extracting solution of enzyme, enzyme concentration, reaction system, reaction duration and termination methods. Finally, we introduced an improved method for detecting activity of cassava starch branching enzyme. Firstly, 0.5 g root of mature cassava was ground into pulp with extracting solution. Then, the crude enzyme solution was obtained from the supernatant after centrifugation. The crude enzyme solution was diluted and added with 0.5% soluble starch at 37℃ water bath for 10 min. Then, the reaction was terminated by boiling water. Iodine solution and KI were added to conduct chromogenic reaction for 10 min. The activity of enzyme was measured at 660 nm. One unit of cassava SBE activity was defined as the amount of substrate causing 1% decrease in absorbance of iodine blue at 660 nm. The improved method is simple and rapid for measuring activity of starch branching enzyme from different cassava germplasms with excellent repeatability. |
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| Keywords: | cassava starch branching enzyme enzyme activity determination method |
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