| 利用RAPD—BSA分子标记技术筛选金针菇色泽基因研究 |
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| 作者姓名： | 刘维侠 谢宝贵 王秀全 江玉姬 |
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| 作者单位： | 中国热带农业科学院热带作物品种资源研究所 海南儋州571737
(刘维侠)，福建农林大学 福建福州350002
(谢宝贵，王秀全)，福建农林大学 福建福州350002(江玉姬) |
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| 基金项目： | 国家科技基础条件平台项目(2004DK30420)，福建省自然科学基金资助项目(B0210020)资助 |
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| 摘 要： | 色泽是金针菇的重要性状之一,受一对等位基因的控制。以FL19(黄色)、8801(白色)及其后代(F1代、F2代)菌株和相应单核菌株为材料,采用分离群体分组分析(BulkedSegregantAnalysis,BSA)方法,对金针菇基因组DNA进行RAPD分析,通过对200个引物的筛选,获得了一个与白色性状基因紧密连锁的RAPD标记S3751800,并在亲本及其后代菌株进行了验证,具有较好的重复性和稳定性。可用于白色金针菇新品种的辅助选育。
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| 关 键 词： | 金针菇 色泽基因 RAPD BSA |
Studies on RAPD Markers of Color Gene in Flammulina velutipes |
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| Authors: | Liu Weixi Xie Baogui Wang Xiuquan Jiang Yuji |
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| Institution: | 1.Tropical Crops Germplasm Resources Research Institute, CA TAS, Danzhou Hainan 571737; 2.Fujian Agriculture and Forestry University, Fuzhou Fujian, 350002 |
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| Abstract: | Color of fruit-body is one of the most important traits of Flammulina velutipes,and it is controlled by a pair of alleles. In our studies, the strain FL19(yellow), 8801(white), and their offspring (F1, F2 generations),as well as their monokaryotics were used. RAPD and BSA was employed to detect molecular markers linked to color gene in the Flammulina velutipes. 200 RAPD primers were screened to identify the markers linked to the color gene of Flammulina velutipes. One sensitive and stable marker S3751800 which was tightly associated with the color gene was found from 200 primers. And the marker was confirmed by the strains of parents and their offspring. The marker S3751800 could be used as a useful marker for the molecular assistant selection in white Flammulina velutipes breeding. |
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| Keywords: | Flammulina velutipes Color gene RAPD BSA |
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