| 山羊卵母细胞的体外成熟研究 |
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| 作者姓名： | 赵晓娥 李向臣 高立功 吴月红 山灵 刘凤军 王永胜 张涌 |
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| 作者单位： | 西北农科技大学生物工程研究所 陕西杨凌712100
(赵晓娥，李向臣，高立功，吴月红，山灵，刘凤军，王永胜)，西北农科技大学生物工程研究所 陕西杨凌712100(张涌) |
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| 基金项目： | 国家（863）高技术研究与发展计划项目“家畜体细胞克隆技术体系的建立和应用”（2001AA213081）. |
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| 摘 要： | 1.比较了在卵母细胞成熟液中添加相同浓度(10ng/ml)的表皮生长因子(EGF),胰岛素样生长因子(IGF),EGF IGF后,不同生长因子对卵母细胞体外发育率的影响。结果表明,在成熟培养液中添加EGF后,其卵母细胞的成熟率(70.9%)与对照组(67.3%)相比有所提高,但差异不显著;在成熟培养液中分别添加IGF、EGF IGF,其卵母细胞的成熟率分别为80.0%,82.4%,显著高于对照组卵母细胞的成熟率(67.3%)。2.比较了不同季节采集的卵母细胞在体外成熟的情况。结果发现,12月份到来年的1月份,采集的卵母细胞,其体外成熟率最高,为81.0%;11月份采集的卵母细胞,其体外成熟率次之,为74.1%;3—5月份和9—10月份采集的卵母细胞,其体外成熟率为66.0%、69.6%;6—7月初采集的卵母细胞,其体外成熟率最差,为54.7%。3.比较了OM液pH值对卵母细胞体外成熟的影响。当OM培养液的pH值为7.2和6.8￣7.0时,卵母细胞成熟率的为66.2%和61.0%,显著高于pH≥7.5时的成熟率(50.9%,P<0.05)。
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| 关 键 词： | 羊 卵母细胞 体外成熟 EGF IGF 季节 pH值 |
Mature Culture of Goat oocytes in vitro |
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| Authors: | Zhao Xiao'e Li Xiangchen Gao Ligong Wu YueHong Shan Ling Liu Fengjun Wang Yongsheng Zhang Yong |
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| Institution: | Bio-engineering institute, Northwest Sci-tech University of Agriculture and Foresty, Yangling Shaanxi 712100 |
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| Abstract: | Goat oocytes were cultured in maturing media (OM) adding equal concentrations (10 ng/ml) EGF, IGF, or EGF IGF. The matured rate (80.0%, 82.4%) of goat oocytes in OM adding IGF and 10 ng/ml (EGF IGF ) were higher significantly than in OM and OM adding EGF(70.9%, 67.3%). Senson can influence the matured rate of goat oocytes. The study show, from December to next year January, that the matured rate of goat oocytes is the highest (81.0%); the matured rate of goat oocytes is 74.1% at November; the matured rate of goat oocytes is the lowest (54.7%) from June to July. pH of OM media can influence the matured rate of goat oocytes. When pH of OM is 6.8~7.0 and 7.2, the matured rate of goat oocytes were higher significantly than that pH of OM is alkaline (?7.5). |
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| Keywords: | Goat Oocyte Mature Culture in vitro EGF IGF Senson pH |
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