| 细菌性条斑病侵染水稻抗性相关蛋白研究 |
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| 引用本文： | 李东霄,张国广,郭立佳,李敏,毛倩,汪卫,陈亮.细菌性条斑病侵染水稻抗性相关蛋白研究[J].中国农业科技导报,2010,12(5):62-67. |
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| 作者姓名： | 李东霄 张国广 郭立佳 李敏 毛倩 汪卫 陈亮 |
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| 作者单位： | (1.河南科技学院, 河南 新乡 453003,2.厦门大学生命科学学院, 福建 厦门 361005) |
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| 基金项目： | 国家863计划项目，国家重大科技专项，教育部重点项目 |
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| 摘 要： | 水稻细菌性条斑病由Xanthomonas oryzae pv. oryzicola引起,是目前威胁亚洲稻区水稻生产的重要病害之一。以水稻品种9311为研究对象, 通过差异蛋白质组学方法,研究了病菌侵染48 h后水稻叶片的差异表达蛋白质,通过分析比对、质谱分析以及数据库检索,从中选择了7个上调表达的鉴定蛋白,包括4个水稻LRK类基因,2个NBS-LRR 类基因和1个PR-10基因家族成员基因。并设计相应的PCR引物,从水稻cDNA 中获得相关基因片段做探针进行Northern杂交分析,结果显示,上述基因在接种病原菌12 h或48 h后表达量增加,表明这些蛋白质参与了抗病反应。
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| 关 键 词： | 细菌性条斑病 水稻 蛋白组学 抗病 Northern杂交 |
| 收稿时间： | 2010-07-05 |
| 修稿时间： | 2010-08-14 |
Studies on Rice Resistance-related Proteins in Response to Bacterial Leaf Streak, Xanthomonas Oryzae pv. Oryzicola |
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| LI Dong-xiao,ZHANG Guo-guang,GUO Li-jia,LI Min,MAO Qian,WANG Wei,CHEN Liang.Studies on Rice Resistance-related Proteins in Response to Bacterial Leaf Streak, Xanthomonas Oryzae pv. Oryzicola[J].Journal of Agricultural Science and Technology,2010,12(5):62-67. |
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| Authors: | LI Dong-xiao ZHANG Guo-guang GUO Li-jia LI Min MAO Qian WANG Wei CHEN Liang |
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| Institution: | (1.Henan Institute of Science and Technology, Henan Xinxiang 453003|2.School of Life Sciences, Xiamen University, Fujian Xiamen 361005, China) |
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| Abstract: | Rice bacterial leaf streak (BLS) caused by the pathogen Xanthomonas oryzae pv. oryzicola (Xooc) is one of the major diseases in Asia. In this paper, the protein differently expressed after infected for 48 h by BLS was studied with rice 9311 as material through proteomic approach. Based on the result of protein blast, fingerprints and data search analysis, seven identified proteins up-regulated were selected including four LRK like genes, two NBS-LRR type genes and one PR-10 gene. Relevant primers were designed to amplify the genes from rice cDNA as probe for Northern blotting. The results showed that the mRNA levels of these genes increased significantly after infected by BLS for 12 h and 48 h, indicating these proteins have participated in disease resistance. |
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| Keywords: | bacterial leaf streak rice proteomics disease resistance Northern blotting |
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