Use of a selective medium to study the dispersal of ascospores ofSclerotinia sclerotiorum |
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Authors: | Y Ben-Yephet S Bitton |
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Institution: | 1. Dept. of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel 2. Extension Service, Israel Ministry of Agriculture, Bet She’an, Israel
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Abstract: | A selective medium (potato dextrose agar with pentachloronitrobenzene and streptomycin) was used to assess dispersal of ascospores ofSclerotinia sclerotiorum in the field. This medium inhibited development ofMucor spp. andRhizopus spp. without affecting the germinability ofS. sclerotiorum ascospores or the production of sclerotia. The number of viable ascospores started increasing in December, was maximal from January to February, and decreased sharply in March. Deposition of ascospores on plates was maximal (57%) between 10:00 and 13:00 hours. Seventy-seven to 90% of the ascospores were deposited within the first 100 m from the source of inoculum and the rest were transported farther. |
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