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新孢子虫速殖子灭活苗对沙鼠的免疫保护性试验
引用本文:李文学,鲁承,杜秋明,贾文影.新孢子虫速殖子灭活苗对沙鼠的免疫保护性试验[J].延边大学农学学报,2010,32(1):4-7.
作者姓名:李文学  鲁承  杜秋明  贾文影
作者单位:延边大学农学院,动物医学系,吉林,龙井,133400
基金项目:吉林省科学技术厅资助项目 
摘    要:为验证2种新孢子虫速殖子灭活苗的免疫保护作用,以沙鼠为试验动物,用2种不同佐剂疫苗对沙鼠进行免疫,21 d后用新孢子虫速殖子(1×106/只)进行皮下攻毒,观察死亡情况.试验结果表明,第Ⅰ种疫苗保护率为85%(17/20),第Ⅱ种疫苗保护率为80%(16/20),并在死亡沙鼠组织DNA均扩增出新孢子虫特异的基因片段.

关 键 词:新孢子虫  灭活苗  沙鼠  免疫保护

Neospora tachyzoites inactivated vaccine on gerbils rotective immunity test
LI Wen-xue,LU Cheng,DU Qiu-ming,JIA Wen-ying.Neospora tachyzoites inactivated vaccine on gerbils rotective immunity test[J].Journal of Agricultural Science Yanbian University,2010,32(1):4-7.
Authors:LI Wen-xue  LU Cheng  DU Qiu-ming  JIA Wen-ying
Institution:LI Wen-xue,LU Cheng,DU Qiu-ming,JIA Wen-ying(Veterinary Department,Agricultural College of Yanbian University,Longjing Jilin 133400,China)
Abstract:In order to validate the immunological protection effect of two different inactivated vaccines a-gainst Neosporiasis tachyzoites. The gerbils were vaccinated twice with two different adjuvant vaccine. 21 days later with tachyzoites (1 ×10^6 Per gerbils) subcutaneous infection and observation gerbils information for death. The results showed that the protection rate is 85M (17/20)of the first kind vaccine,the other (Ⅱ)vaccine protection rate 80M (16/20). Amplification Neosporiasis specif fragment from the death gerbils tissue DNA.
Keywords:Neospora  inactivated vaccine  gerbil  immunoprotection  
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