| 家兔魏氏梭菌的分离与半套式PCR鉴定 |
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| 引用本文： | 黄勇,宋宇,刘温泉,范荣香,王远萍,邹年莉.家兔魏氏梭菌的分离与半套式PCR鉴定[J].畜禽业,2008(2):61-62. |
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| 作者姓名： | 黄勇 宋宇 刘温泉 范荣香 王远萍 邹年莉 |
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| 作者单位： | 四川农业大学动物医学院,四川,雅安,625014 |
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| 摘 要： | 魏氏梭菌病是危害养兔业的重要疾病。某养兔场发生一种以急性黑色水样腹泻或排带血的胶冻样粪便、盲肠出血、胃粘膜出血为主要特征的传染病,通过病原菌的分离培养、形态染色观察、生化鉴定和半套式PCR反应,分离出一株魏氏梭菌。通过药物敏感实验筛选出了敏感药物。实验结果为该病的正确防控措施的提出提供了参考。
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| 关 键 词： | 魏氏梭菌 分离 鉴定 半套式 PCR |
| 文章编号： | 1008-0414（2008）02-0061-02 |
| 收稿时间： | 2007-11-19 |
| 修稿时间： | 2007年11月19 |
The isolation and half-nested PCR identification of Rabbits Clostridium perfringens |
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| HUNANG Yong,SONG Yu,LIU Wen-quan,FAN Rong-xiang,WANG Yuan-ping,ZOU Nian-li.The isolation and half-nested PCR identification of Rabbits Clostridium perfringens[J].Livestock and Poultry Industry,2008(2):61-62. |
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| Authors: | HUNANG Yong SONG Yu LIU Wen-quan FAN Rong-xiang WANG Yuan-ping ZOU Nian-li |
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| Abstract: | Rabbit clostridial disease is an important disease that threat rabbit production. An acute rabbit infectious disease characteristic of dark diarrhea or gumLike blood feces and cecum bleeding and stomach mucosa bleeding occurrencd in a rabbit farm. A Clostridium perfringens strain was isolated through pathogen isolation, morphologic observation, biochemical reaction and half-nested PCR reaction. Sensive drug were selected by drug sensive experiment. This experiment resuLt provide reference for the establishment of precaution measure of this disease. |
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| Keywords: | Clostridium perfringens Isolation Identification half-nested PCR |
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