Incidence and avoidance of zona fracture in cryopreserved bovine ova and embryos |
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| Authors: | Subramaniam A Devarajan K P Mohanan M |
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| Institution: | Embryo Transfer State Centre, Periyar District Co-operative Milk Producers' Union Ltd, Vasavi College Post, Erode 638 316, Tamil Nadu. |
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| Abstract: | Ova (n=62), which were collected from slaughterhouse bovine ovaries, and embryos (n=26), which were non-surgically recovered from 11 superovulated crossbred donor cows, were frozen. The frozen ova and embryos were then thawed using two conventional thawing protocols, i.e. at 37 degrees C for 30 seconds in a water bath and at 25 degrees C for 2 minutes in air. Some 64.5% of the ova and 53.8% of the embryos thawed in the water bath and 16.1% of the ova and 7.7% of the embryos thawed in ambient air exhibited fractured zonae pellucidae. The slow thawing protocol had a lower incidence of zona damage in cryopreserved oval and embryos than the fast thawing protocol. A low pregnancy rate (12.5%) was recorded for embryos transferred with zona fracture while embryos transferred with intact zonae had a rate of 35.3%) indicating that embryos with zona damage are less viable. |
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