| 西南桦硅胶干燥叶DNA提取方法比较 |
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| 引用本文： | 董蔚,黄寿先,陈荣,桂仁意.西南桦硅胶干燥叶DNA提取方法比较[J].西南林学院学报,2010,30(4):36-38,43. |
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| 作者姓名： | 董蔚 黄寿先 陈荣 桂仁意 |
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| 作者单位： | 1. 浙江林学院,亚热带森林培育国家重点实验室培育基地,浙江,临安,311300
2. 广西大学,林学院,广西,南宁,530004 |
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| 基金项目： | 广西大学中南速生材繁育实验室开放研究课题 |
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| 摘 要： | 采用常规CTAB法、CTAB硅珠法、核分离法、二次抽提法、改良CTAB法对富含多糖的西南桦干叶进行DNA提取,并将DNA进行电泳检测及RAPD分析,结果表明：不同提取方法结果差异较大,CTAB硅珠法得率最低,为0.4～11.6μg/g;核分离法和二次抽提法较常规法去除多糖效果好,得率高;改良CTAB法得率最高,为171.2～364.8μg/g,电泳条带最清晰,RAPD扩增结果最好,适合干叶西南桦基因组DNA提取。
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| 关 键 词： | 干叶 DNA提取 RAPD 西南桦 |
Comparison on DNA Extraction Methods from Betula alnoides Leaves Dried with Silica Gel |
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| DONG Wei,HUANG Shou-xian,CHEN Rong,GUI Ren-yi.Comparison on DNA Extraction Methods from Betula alnoides Leaves Dried with Silica Gel[J].Journal of Southwest Forestry College,2010,30(4):36-38,43. |
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| Authors: | DONG Wei HUANG Shou-xian CHEN Rong GUI Ren-yi |
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| Institution: | DONG Wei1,HUANG Shou-xian2,CHEN Rong1,GUI Ren-yi1(1.A Nurturing Station for the State Key Laboratory of Subtropical Silviculture,Zhejiang Agriculture and Forestry University,Lin'an Zhejiang 311300,China,2.Forestry College,Guangxi University,Nanning Guangxi 530004,China) |
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| Abstract: | The total genomic DNA was extracted from the dried leaves of Betula alnoides with CTAB,CTAB-SiO2,the nuclear separation,double times extraction,and modified CTAB methods,and the DNA obtained was analyzed by the means of agarose gel electrophoresis and RAPD analysis.The results showed that there was great difference in DNA yield from different extraction methods.The DNA yield by CTAB-SiO2 method was the lowest with 0.4-11.6 μg/g.The DNA yield obtained by the nuclear separation and double times extraction methods was higher than the CTAB method,which could eliminate the glucose more efficiently.The DNA yield from the modified CTAB method was the highest,which was 171.2-364.8 μg / g,the polymorphic patterns amplified by RAPD were the best with clear electrophoresis belts.It was concluded that the modified CTAB method was suitable to extract the total genomic DNA from dried leaves of Betula alnoides. |
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| Keywords: | RAPD |
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