| 抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用 |
| |
| 引用本文： | 殷得所,夏明元,李进波,万丙良,査中萍,杜雪树,戚华雄.抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用[J].中国水稻科学,2011,25(1):25-30. |
| |
| 作者姓名： | 殷得所 夏明元 李进波 万丙良 査中萍 杜雪树 戚华雄 |
| |
| 作者单位： | 湖北省农业科学院 粮食作物研究所, 湖北 武汉 430064; *通讯联系人， E-mail: qihx@hbaas.com |
| |
| 摘 要： | 利用带有广谱抗稻瘟病基因Pi9的籼稻品系75 1 127作为抗病基因供体亲本，用于扬稻6号和R6547抗病性的回交育种。通过比较75 1 127、扬稻6号和日本晴的Pi9基因位点的DNA序列，开发出了与Pi9基因紧密连锁的共显性STS（序列标记位点）标记PB9 1，用于Pi9基因的分子标记辅助选择。结合田间农艺性状选择和分子标记辅助选择，培育出8个Pi9基因纯合的回交后代株系。其中，具有扬稻6号和R6547遗传背景的株系各4个。经湖北恩施和宜昌的病圃鉴定，携带有抗病基因株系的稻瘟病抗性水平较受体品种扬稻6号和R6547有不同程度的提高。具有R6547遗传背景的株系08C893配制的杂交组合在上述病区的抗性表现也明显优于对照品种扬两优6号。上述结果说明，共显性标记PB9 1在Pi9抗稻瘟病基因分子标记辅助育种中具有应用价值，并且Pi9基因作为稻瘟病抗源之一可以在湖北稻区进行有效利用。
|
| 关 键 词： | 稻瘟病抗性 分子标记辅助选择 序列标记位点 抗性基因 育种 水稻 |
| 收稿时间： | 1900-01-01; |
Development of STS Marker Linked to Rice Blast Resistance Gene Pi9 in Marker Assisted Selection Breeding |
| |
| YIN De-suo,XIA Ming-yuan,LI Jin-bo,WAN Bing-liang,ZHA Zhong-ping,DU Xue-shu,QI Hua-xiong.Development of STS Marker Linked to Rice Blast Resistance Gene Pi9 in Marker Assisted Selection Breeding[J].Chinese Journal of Rice Science,2011,25(1):25-30. |
| |
| Authors: | YIN De-suo XIA Ming-yuan LI Jin-bo WAN Bing-liang ZHA Zhong-ping DU Xue-shu QI Hua-xiong |
| |
| Institution: | Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; *Corresponding author, E-mail: qihx@hbaas.com |
| |
| Abstract: | Indica rice line 75 1 127 carrying broad spectrum rice blast resistance gene Pi9 was used as resistance gene donor for genetic improvement of Yangdao 6 and R6547. By alignment of genomic sequences from the Pi9 locus of 75 1 127, Yangdao 6 and Nipponbare, co dominant STS (sequence tagged site) marker PB9 1, which linked to the Pi9 gene, was developed for molecular marker assisted selection (MAS). Eight backcross lines carrying Pi9 were obtained, among which four lines contained the genetic background of Yangdao 6 and the other four lines had the R6547 background. Under natural disease infection conditions in Enshi County and Yichang County of Hubei Province, China, the Pi9 carrying lines showed higher blast disease resistance than Yangdao 6 and R6547 in various degrees. The hybrids originated from 08C893, an R6547 derived rice line, showed higher blast disease resistance as compared to the control Yangliangyou 6. The results suggest PB9 1 is an effective co dominant marker for MAS of Pi9 which is a valuable resistance gene for the control of rice blast disease in Hubei area. |
| |
| Keywords: | blast resistance molecular marker assisted selection sequence tagged site resistance gene breading rice |
|
| 点击此处可从《中国水稻科学》浏览原始摘要信息 |
| 点击此处可从《中国水稻科学》下载免费的PDF全文 |
|
