| 磨盘和富有柿果实ACS基因片段的克隆 |
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| 引用本文： | 马俊莲,唐霞,刘月英,刘凯,张子德.磨盘和富有柿果实ACS基因片段的克隆[J].河北农业大学学报,2004,27(4):40-44. |
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| 作者姓名： | 马俊莲 唐霞 刘月英 刘凯 张子德 |
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| 作者单位： | 1. 河北农业大学食品学院,河北,保定,071001
2. 河北经贸大学生物科学与工程学院,河北,石家庄,050061 |
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| 摘 要： | 利用RT-PCR的方法，从磨盘、富有柿果实中克隆了ACC合成酶(ACS)的3个DNA片段。序列分析表明：Mopan-ACS1、Mopan-ACS2与平核无柿果实DK-ACS1的核苷酸同源性为75．6％，氨基酸同源性分别为98.7％和98.2％。Fuyu-ACS与DK-ACS1的核苷酸同源性为99．2％，氨基酸的同源性为99．4％。3个片段编码的氨基酸序列中，均含有植物ACS的保守氨基酸区和不变氨基酸残基。
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| 关 键 词： | 柿果 磨盘 富有 ACS 基因克隆 |
| 文章编号： | 1000-1573(2004)04-0040-05 |
Cloning of ACC sythase from Mopan and Fuyu persimmon fruits |
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| MA Jun-lian,TANG Xia,LIU Yue-ying,LIU Kai,ZHANG Zi-de.Cloning of ACC sythase from Mopan and Fuyu persimmon fruits[J].Journal of Agricultural University of Hebei,2004,27(4):40-44. |
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| Authors: | MA Jun-lian TANG Xia LIU Yue-ying LIU Kai ZHANG Zi-de |
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| Institution: | MA Jun-lian~1,TANG Xia~1,LIU Yue-ying~2,LIU Kai~1,ZHANG Zi-de~1 |
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| Abstract: | Three fragments of ACS were cloned from Mopan and Fuyu persimmon fruits by RT-PCR. Sequence analysis indicated that nucleotide identities of Mopan-ACS1 and Mopan-ACS2 with DK-ACS1 were 76.5%, and amino acid identities were 98.7% and 98.2% respectively. Identities of nucleotide and amino acid between Fuyu-ACS and DK-ACS1 were 99.2% and 99.4% respectively. Mopan-ACS1, Mopan-ACS2 and Fuyu-ACS have the conserved amino acid regions and invariant amino acid residues of ACC synthase existing in other plants. |
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| Keywords: | persimmon fruit Mopan Fuyu ACS DNA cloning |
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