Cloning,expression and functional analysis of HMW glutenin subunit 1By8 gene from Italy pasta wheat (Triticum turgidum L. ssp. durum) |
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| Institution: | 1. Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, 100037 Beijing, China;2. Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing 100081, China;3. CIMMYT-China Office, c/o CAAS, Beijing 100081, China;4. Western Australian Department of Agriculture and Food; Centre for Comparative Genomics and State Agriculture Biotechnology Centre, Murdoch University, Perth, WA 6150, Australia;5. CSIRO Plant Industry, Canberra, Australia;1. USDA-ARS Western Wheat Quality Laboratory, E-202 Food Quality Building, Washington State University, Pullman, WA, 99164-6394, USA;2. Quality Assurance Manager, Firestone Pacific Foods, Vancouver, WA 98660, USA;3. Formerly School of Food Science, Washington State University, Pullman, WA, 99164, USA;4. Phytelligence Inc, Pullman, WA, USA;5. Formerly Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164, USA;1. The University of Sydney Plant Breeding Institute-Cobbitty, PMB 4011, Narellan, NSW 2567, Australia;2. Environment and Biotechnology Centre, Faculty of Life and Social Sciences, Swinburne University of Technology, PO Box 218, Melbourne, Victoria 3122, Australia;1. Advanced Plant Technology Program, Clemson University, 105 Collings St., Clemson, SC, 29634-0141, USA (formerly Western Wheat Quality Laboratory);2. USDA-ARS Western Wheat Quality Laboratory, E-202 Food Quality Building, Washington State University, Pullman, WA, 99164-6394, USA;3. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164, USA;1. Plant Genetic Resources Centre, National Institute for Agricultural and Food Research and Technology, Autovía de Aragón Km 36, 28800, Alcalá de Henares, Spain;2. Department of Biotechnology-Plant Biology, School of Agricultural Engineering, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040 Madrid, Spain;1. Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China;2. International Maize and Wheat Improvement Center (CIMMYT), c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China;3. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, 4072, Australia;4. International Maize and Wheat Improvement Center (CIMMYT), Yenimahalle, Ankara, 06170, Turkey;5. Department of Plant Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan;1. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA;2. Nutrition & Food Science Department, Texas A&M University, College Station, TX 77843, USA;3. USDA-ARS Center for Grain and Animal Health Research, Manhattan, KS 66502, USA |
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| Abstract: | Cloning and functional analysis of high molecular weight wheat glutenin subunit (HMW-GS) 1By8 from Italy durum cultivar Simeto was carried out in this study. All HMW-GS from Simeto were separated and characterized by appropriate electrophoresis methods, reversed-phased high performance liquid chromatography (RP-HPLC) and mass spectrometry (MS). The complete gene encoding 1By8 subunit was amplified by allele-specific PCR primers, including an upstream sequence of 857 bp and an open reading frame (ORF) of 2166 bp encoding a mature protein of 720 amino acid residues. The promoter sequence, containing -300 element (cereal glutenin gene control element) and enhancer was highly conserved among HMW-GS genes. Comparison with the sequence of subunit 1By9 from bread wheat demonstrated 99% identity with the main difference being that the 1By8 subunit possesses an additional insertion of 15 amino acid residues (QYPASQQQPA QGQQG) at position 342 and two residue substitutions at position 78 (leucine/proline) and 442 (arginine/glutamine). The molecular weight differences between MALDI-TOF-MS and deduced amino acid sequence of the coding gene revealed the possibility of some kinds of post-translational modifications present in 1By8 subunit. The protein subunit expressed in Escherichia coli showed a very similar mobility to the endogenous 1By8 of Simeto on SDS-PAGE. The function of the isolated protein on wheat processing quality was determined by 10 g Mixgraph analysis. Results demonstrated that addition of y-type HMW glutenin subunits into the base flour had significant positive effects on main mixing parameters and significant difference in effects were observed among different y-type subunits. |
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