利用噬菌体随机肽库筛选NDV单克隆抗体识别的抗原表位 |
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引用本文: | 姜宁朋,胡北侠,张秀美,黄艳艳,李建亮,崔言顺.利用噬菌体随机肽库筛选NDV单克隆抗体识别的抗原表位[J].农业生物技术学报,2008,16(6). |
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作者姓名: | 姜宁朋 胡北侠 张秀美 黄艳艳 李建亮 崔言顺 |
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作者单位: | 山东农业大学动物医学院 山东农业科学院畜牧兽医研究所 |
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摘 要: | 为了获得新城疫病毒(NDV)HN蛋白单克隆抗体1E5识别的抗原位点, 以NDV LaSota株HN蛋白的单克隆抗体(mAb)1E5为靶分子,采用噬菌体随机7肽库进行亲和筛选,并用间接ELISA和竞争ELISA鉴定阳性克隆。最终得到4组可用的7肽序列,同源性分析表明展示肽序列与HN蛋白的388~395氨基酸具有较高的同源性,所以它们的共有序列L * * * PNT是抗原表位的骨架结构。这个表位的位置与以往的文献报道不同,它很可能是新城疫病毒HN蛋白的另一个重要的抗原位点。
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关 键 词: | 新城疫病毒 噬菌体随机肽库 单克隆抗体 抗原表位 |
收稿时间: | 2007-11-12 |
修稿时间: | 2008-6-16 |
Identification of the Simulative Epitope of NDV Monoclonal Antibody with Phage Displayed Random Peptide Library |
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Abstract: | To get the epitope site of NDV-HN protein which may be identified with mAb 1E5, monoclonal antibody of NDV-HN protein was used to screen the phage display random 7-peptides. The positive clonies were identified by indirect ELISA and competitive ELISA. 4 groups of 7-peptides sequence were got These sequences were located at the residue 388~395 aa of NDV-HN protein.The mutual sequence L * * * PNT is the framework structure. This epitope site is different with former reports, It may be another useful epitope site of NDV-HN protein. |
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