| 越冬期体色变异红罗非鱼差异表达miRNA的鉴定 |
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| 引用本文： | 王兰梅,朱文彬,傅建军,罗明坤,董在杰.越冬期体色变异红罗非鱼差异表达miRNA的鉴定[J].水产学报,2023,47(4):049602-049602. |
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| 作者姓名： | 王兰梅 朱文彬 傅建军 罗明坤 董在杰 |
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| 作者单位： | 中国水产科学研究院 淡水渔业研究中心,中国水产科学研究院 淡水渔业研究中心,中国水产科学研究院 淡水渔业研究中心,中国水产科学研究院 淡水渔业研究中心,中国水产科学研究院 淡水渔业研究中心 |
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| 基金项目： | 国家自然科学基金青年基金(31802290)和中国水产科学研究院基本科研业务费项目（2020TD37） |
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| 摘 要： | 为了探究红罗非鱼越冬期体色变异的分子机制，实验构建了其2种体色(正常粉红体色和变黑体色)皮肤组织的小RNA文库，每组4个重复，组装后平均每组分别获得12 190 544和11 891 890条过滤后的读长(clean reads)，获得miRNA成熟序列669个，其中337个是已知的miRNAs。鉴定出越冬期变黑体色相对于正常粉红体色鱼的差异表达miRNAs 26个，其中上调11个，下调15个，且12个为已知的miRNAs，可能在红罗非鱼越冬期体色变异过程中发挥重要作用。富集分析发现了大量代谢和体色调控相关的通路。研究表明，红罗非鱼越冬期的体色变异可能是色素细胞的增殖和迁移形成的，与代谢相关的调控通路在其中发挥重要作用，为解析红罗非鱼越冬期体色变异的机制奠定了基础。
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| 关 键 词： | 红罗非鱼 越冬 体色变黑 microRNA (miRNA) |
| 收稿时间： | 2022/1/18 0:00:00 |
| 修稿时间： | 2022/3/23 0:00:00 |
Identification of differentially expressed miRNA involved in skin color variation in red tilapia during overwintering |
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| WANG Lanmei,ZHU Wenbin,FU Jianjun,LUO Mingkun,DONG Zaijie.Identification of differentially expressed miRNA involved in skin color variation in red tilapia during overwintering[J].Journal of Fisheries of China,2023,47(4):049602-049602. |
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| Authors: | WANG Lanmei ZHU Wenbin FU Jianjun LUO Mingkun DONG Zaijie |
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| Institution: | F Freshwater Fisheries Research Centre of Chinese Academy of Fishery Sciencesreshwater Fisheries Research Centre of Chinese Academy of Fishery Sciences,Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Centre of Chinese Academy of Fishery Sciences,Freshwater Fisheries Research Centre of Chinese Academy of Fishery Sciences,Freshwater Fisheries Research Centre of Chinese Academy of Fishery Sciences,Freshwater Fisheries Research Centre of Chinese Academy of Fishery Sciences |
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| Abstract: | Red tilapia (Oreochromis spp.) is a valuable fish due to its uniform red skin, the absence of black peritoneum, very fast growth and adaptability to any culture system, and it has a huge market in many parts of the world, such as China, Malaysia and Thailand. However, the skin color differentiation in genetic breeding a nd skin color variation (whole pink to black) during the overwintering period are the main problems limiting the development of commercial red tilapia culture. MicroRNAs (miRNAs) play important roles in biological processes by regulating specific gene expression. Limited microRNA (miRNA) information is available on skin color variation in fish so far. In this study, a high-throughput Illumina sequencing of sRNAs was conducted on two color varieties of red tilapia during the overwintering. The two skin color varieties were normal whole pink (WP) and pink to black color (PB). A mean of 14,748,358 and 12,981,453 raw reads from WP and PB group, respectively, corresponding to 12,190,544 and 11,891,890 clean reads. We summarized the length distribution of all samples and showed that the peak length was 22nt. A total of 669 mature miRNAs were identified, including 337 known miRNAs and 332 novel miRNAs. Moreover, we identified 26 significant differential expression miRNAs in PB compared with WP group, including 11 up-regulated miRNAs and 15 down-regulated miRNAs. And 12 known miRNAs including miR-203a, miR-200a, miR-200b, miR-725, miR-10544, miR-204a, miR-429b, miR-129, miR-10581c, miR-10581a, miR-217 and miR-499 may play an important role in the skin color variation of red tilapia during overwintering. Enrichment analysis found many pathways related to metabolic and skin color regulation including oocyte meiosis, Wnt, Notch, calcium, Apelin signaling pathway and so on. These results would provide a basis for the mechanism of skin color variation of red tilapia during overwintering. |
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| Keywords: | Red tilapia overwintering skin color black microRNA (miRNA)ed tilapia overwintering skin color black microRNA (miRNA) |
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