| 不同种型布鲁菌标准株的比较基因组学研究 |
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| 引用本文： | 钟志军,徐杰,于爽,王玉飞,周冬生,黄祥明,乔凤,曲勍,杨洋,罗永久,刘学涵,原慧,陈燕芬,黄克和,陈泽良,彭广能.不同种型布鲁菌标准株的比较基因组学研究[J].兽医大学学报,2013(12):1783-1791. |
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| 作者姓名： | 钟志军 徐杰 于爽 王玉飞 周冬生 黄祥明 乔凤 曲勍 杨洋 罗永久 刘学涵 原慧 陈燕芬 黄克和 陈泽良 彭广能 |
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| 作者单位： | [1]四川农业大学动物医学院,四川雅安625014 [2]军事医学科学院疾病预防控制所,北京100071 [3]军事医学科学院微生物流行病研究所,北京100071 [4]成都大熊猫繁育研究基地,四川省濒危野生动物保护生物学重点实验室-省部共建国家重点实验室培育基地,四川成都610081 [5]南京农业大学动物医学院,江苏南京210095 |
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| 基金项目： | 基金项目：国家973课题（2009CB522602）;国家自然科学基金资助项目（31000548）;教育部“长江学者和创新团队发展规划”创新团队资助项目（IRT0848）;四川农业大学双支计划资助项目（00270713）;成都大熊猫繁育研究基金会资助项目（CPF研2012-6）;四川省教育厅重点资助项目（13ZA0263）’ |
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| 摘 要： | 探究不同种型布鲁菌标准株的基因组构成差异。通过布鲁菌全基因组DNA芯片技术对19株不同种型布鲁菌标准株进行比较基因组学研究。结果显示：19株不同种型布鲁菌标准株之间存在大量缺失基因，同时发现一些基因以多拷贝形式存在。缺失基因的功能大致分为4类：信息储存和传递；胞内活动处理；营养代谢、功能未知或仅了解部分功能，共鉴定到这类基因211个。深入认识了19株不同种型布鲁茵标准株基因组组成上的差异，为我们进一步认识不同种以及亚型在毒力以及宿主特殊性提供了依据。大量缺失基因在19株布鲁菌标准株出现，构成了布鲁菌标准株不同种以及亚型之间的遗传学基础。
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| 关 键 词： | 布鲁菌标准株不同种型 比较基因组学 |
Comparative genomics research on different Brucella species and biovars |
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| Institution: | ZHONG Zhi-jun, XU Jie , YU Shuang , WANG Yu-fei , ZHOU Dong-sheng , HUANG Xiang- ming ,QIAO Feng ,QU Qing ,YANG Yang ,LUO Yong-jiu ,LIU Xue-han ,YUAN Hui ,CHEN Yan-fen , HUANG Ke-he , CHEN Ze-liang , PENG Gang-neng (1. College of Veterinary Med- icine, Sichuan Agricultural University, Ya an sichuan 625014, China ; 2. Institute of Disease Con- trol and Prevention ,Academy of Military Medical Science, Beijing 100071, China ; 3. Beijing In- stitute of Microbiology and Epidemiology , State Key Laboratory of Pathogen and Biosecurity , Beijing 100071 ,China;4. Chengdu Research Base of Giant Panda Breeding,key laboratory of bi- ology to protect endangered wild animal sichuan, State Key Laboratory for China, Chengdu 610081, China; 5. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China ) |
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| Abstract: | Nineteen type strains of Brucella were analyzed by comparative genomics hybridization to explore the genetic variation between different Brucella species and biovars. Results demonstra- ted that there were a lot missing gene and several genes were found to be multi-copied in 19 type strains. Functions of the gene （identified 211） were divided into four categories （information stor- age and processing, cellular processes metabolism, poorly characterized or only understand part function in nutritional and metabolic function. The differences between different Brucella species and biovars were in-depth understanding. Our results are useful to more understanding virulence and host particularity in different species and biovars. A large number of missing gene in 19 type strains of Brucella appears to constitute the genetic variations between different species andbiovars of the standard strains of Brucella. |
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| Keywords: | different Brucella species and biovars comparative genomics |
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