| 羊布鲁菌16MOmp25真核表达载体的构建 |
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| 引用本文： | 屈海龙,王海浪,陈思,张瑞,王秀然,钱晶,王兴龙.羊布鲁菌16MOmp25真核表达载体的构建[J].兽医大学学报,2013(12):1848-1850,1854. |
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| 作者姓名： | 屈海龙 王海浪 陈思 张瑞 王秀然 钱晶 王兴龙 |
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| 作者单位： | [1]军事医学科学院军事兽医研究所,长春130122 [2]吉林省人兽共患病预防与控制重点实验室,长春130122 [3]北京奶牛中心,北京100192 |
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| 基金项目： | 基金项目：国家自然科学基金资助项目（30972198/C180503）;国家科技支撑计划（2010BAD04803） |
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| 摘 要： | 利用聚合酶链式反应（PCR），以羊布鲁菌16M基因组为模板克隆Omp25基因，设计含有EcoRI和xhoI酶切位点的引物序列，定向克隆到真核表达载体pCMV-HA上，构建真核表达重组质粒pCMV-HA～Omp25。经测序及双酶切验证正确，证明成功构建了pCMV-HA—Omp25真核表达质粒，为表达纯化外膜蛋白Omp25，研究其功能奠定基础。
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| 关 键 词： | 羊布鲁菌16M Omp25 真核表达 |
The construction of Brucella melitensis 16M Omp25 gene in eukaryotic expression vector |
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| Authors: | QU Hai-long WANG Hai-lang CHEN Si ZHANG Rui WANG Xiu-ran QIAN Jing WANG Xing-long |
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| Institution: | 1. The Military Veterinary Institute ,Academy of Military Medical Sci- ences of PLA ,Changchun 130122 ,China ; 2. Key Laboratory of Jilin Province for Zoonosis Pre- vention and Control, Changchun 130122, China ; 3. Beijing Dairy Cattle Center, Beijing 100192, China ) |
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| Abstract: | In this research,Brucella rnelitensis 16M Omp25 gene was amplified by PCR. The puri- fied PCR products were ligated with pMD18-T simple vector. After enzyme digestion and sequence analysis,the correct pMD-18T-Omp25 gene was digested by EcoR I and Xho,and cloned into the eukaryotic expression vector pCMV-HA which was digested by the same enzymes. The result shows that the eukaryotic expression vector of Brucella rnelitensis 16M Omp25 gene was con- structed successfully,which laid the foundation for the Omp25 function analysis. |
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| Keywords: | Brucella rn elitensis 16 M Omp2 5 eukaryotic expression |
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